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J Appl Physiol 69: 1546-1548, 1990;
8750-7587/90 $5.00
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Journal of Applied Physiology, Vol 69, Issue 4 1546-1548, Copyright © 1990 by American Physiological Society

ARTICLES

The acoustocardiogram: a noninvasive method for measuring heart rate of avian embryos in ovo

H. Rahn, S. A. Poturalski and C. V. Paganelli
Department of Physiology, State University of New York, Buffalo 14214.

A method is presented for measuring the heart rate of avian eggs noninvasively during the last half of incubation. The technique involves briefly placing an egg in tightly sealed vessel containing an inexpensive condenser microphone. The amplified output of the microphone, termed the acoustocardiogram (ACG), is nearly sinusoidal in shape and synchronous with the electrocardiogram. The ACG can also be obtained by mounting the microphone directly on the shell with Plasticine. The method offers advantages over previously described techniques in simplicity, low cost, and noninvasiveness.


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