Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 68: 1854-1864, 1990;
8750-7587/90 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heidelberger, E.
Right arrow Articles by Reeves, R. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heidelberger, E.
Right arrow Articles by Reeves, R. B.

Journal of Applied Physiology, Vol 68, Issue 5 1854-1864, Copyright © 1990 by American Physiological Society

ARTICLES

O2 transfer kinetics in a whole blood unicellular thin layer

E. Heidelberger and R. B. Reeves
Department of Physiology, School of Medicine, State University of New York, Buffalo 14214.

A planar monocellular layer of whole blood (WB) sandwiched between two Gore-Tex membranes is used to study O2 uptake and release kinetics at 37 degrees C. Gore-Tex, a highly gas-permeable open mesh of Teflon fibrils (78% porosity, 0.2-microns pore size, 75-microns thick), constrains WB to form a thin film without imposing an appreciable gas diffusion barrier. WB layer thickness, measured by isotope dilution, is 1.7 +/- 0.2 microns. WB films are mounted between fiber optics in a gas flow tube for dual-wavelength (536/558 nm) oxyhemoglobin saturation measurements after a step change in PO2. For isocapnic (6% CO2) step changes in PO2 between 0 and 104 Torr, WB O2 uptake half time is 10.4 +/- 0.9 ms; WB O2 release half time is 20.6 +/- 2.4 ms. Half-time values are half of those previously reported. The thin-layer method reduces erythrocyte diffusion boundary layer error and thereby offers an attractive alternative to classical rapid fluid-mixing techniques.


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
S. Chakraborty, V. Balakotaiah, and A. Bidani
Diffusing capacity reexamined: relative roles of diffusion and chemical reaction in red cell uptake of O2, CO, CO2, and NO
J Appl Physiol, December 1, 2004; 97(6): 2284 - 2302.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
H. Heller and K.-D. Schuster
Theta values for C16O18O and C18O2 related to respective pulmonary diffusing capacities
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 1998; 274(5): R1496 - R1499.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
H. Heller, G. Fuchs, and K.-D. Schuster
Pulmonary diffusing capacities for oxygen-labeled CO2 and nitric oxide in rabbits
J Appl Physiol, February 1, 1998; 84(2): 606 - 611.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online