Journal of Applied Physiology, Vol 68, Issue 4 1581-1589, Copyright © 1990 by American Physiological Society

ARTICLES

Derecruitment of filtration surface area in paraquat-injured isolated dog lungs

T. Shibamoto, J. C. Parker, A. E. Taylor and M. I. Townsley
Department of Physiology, College of Medicine, University of South Alabama, Mobile 36688.

The capillary filtration coefficient (Kf,c) is a sensitive and specific index of vascular permeability if surface area remains constant, but derecruitment might affect Kf,c in severely damaged lungs with high vascular resistance. We studied the effect of high and low blood flow rates on Kf,c in papaverine-pretreated blood-perfused isolated dog lungs perfused under zone 3 conditions with and without paraquat (PQ, 10(-2) M). Three Kf,cs were measured successively at hourly intervals for 5 h. These progressed sequentially from isogravimetric blood flow with low vascular pressure (I/L) to high flow with low vascular pressure (H/L) to high flow with high vascular pressure (H/H). The blood flows of H/L and H/H were greater than or equal to 1.5 times that of I/L. There were no significant changes in Kf,c in lungs without paraquat over a 50-fold range of blood flow rates. At 3 h after PQ, I/L-Kf,c was significantly increased and both isogravimetric capillary pressure and total protein reflection coefficient were decreased from base line. At 4 and 5 h, H/L-Kf,c was significantly greater than the corresponding I/L-Kf,c (1.01 +/- 0.22 vs. 0.69 +/- 0.09 and 1.26 +/- 0.19 vs. 0.79 +/- 0.10 ml.min-1.cmH2O-1.100 g-1, respectively) and isogravimetric blood flow decreased to 32.0 and 12.0% of base line, respectively. Pulmonary vascular resistance increased to 12 times base line at 5 h after PQ. We conclude that Kf,c is independent of blood flow in uninjured lungs. However, Kf,c measured at isogravimetric blood flow underestimated the degree of increase in Kf,c in severely damaged and edematous lungs because of a high vascular resistance and derecruitment of filtering surface area.

This article has been cited by other articles:

				T. Miyahara, K. Hamanaka, D. S. Weber, D. A. Drake, M. Anghelescu, and J. C. Parker Phosphoinositide 3-kinase, Src, and Akt modulate acute ventilation-induced vascular permeability increases in mouse lungs Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L11 - L21. [Abstract] [Full Text] [PDF]

				J. C. Parker and M. I. Townsley Evaluation of lung injury in rats and mice Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L231 - L246. [Abstract] [Full Text] [PDF]

				D. F. Alvarez, E.-A. B. Gjerde, and M. I. Townsley Role of EETs in regulation of endothelial permeability in rat lung Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L445 - L451. [Abstract] [Full Text] [PDF]

				J. C. Parker Inhibitors of myosin light chain kinase and phosphodiesterase reduce ventilator-induced lung injury J Appl Physiol, December 1, 2000; 89(6): 2241 - 2248. [Abstract] [Full Text] [PDF]

				P. M. Becker, W. Buchanan, and J. T. Sylvester Protective effects of intravascular pressure and nitric oxide in ischemic lung injury J Appl Physiol, March 1, 1998; 84(3): 803 - 808. [Abstract] [Full Text] [PDF]

				D. Anglade, M. Corboz, A. Menaouar, J. C. Parker, S. Sanou, S. Bayat, G. Benchetrit, and F. A. Grimbert Blood flow vs. venous pressure effects on filtration coefficient in oleic acid-injured lung J Appl Physiol, March 1, 1998; 84(3): 1011 - 1023. [Abstract] [Full Text] [PDF]

				T. Miyahara, T. Shibamoto, H.-G. Wang, and S. Koyama Role of circulating blood components and thromboxane in anaphylactic vasoconstriction in isolated canine lungs J Appl Physiol, November 1, 1997; 83(5): 1508 - 1516. [Abstract] [Full Text] [PDF]