Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 68: 1481-1485, 1990;
8750-7587/90 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ianuzzo, C. D.
Right arrow Articles by Chiu, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ianuzzo, C. D.
Right arrow Articles by Chiu, R.

Journal of Applied Physiology, Vol 68, Issue 4 1481-1485, Copyright © 1990 by American Physiological Society

ARTICLES

Biochemical transformation of canine skeletal muscle for use in cardiac-assist devices

C. D. Ianuzzo, N. Hamilton, P. J. O'Brien, C. Desrosiers and R. Chiu
Department of Biology and Physical Education, York University, North York, Ontario, Canada.

Skeletal muscle has an inherent biochemical phenotypic plasticity that provides the possibility for it to be remodeled into a "heart-like" muscle for use in cardiac-assist devices. The purpose of this study was to chronically stimulate skeletal muscle electrically to transform the biochemical capacities of the three major subcellular systems (i.e., metabolic, calcium regulating, and contractile) to resemble those of heart muscle. The latissimus dorsi muscle (LDM) of mongrel dogs weighing 22-27 kg was stimulated via the thoracodorsal nerve at 2 Hz for 6-8 wk. This stimulation protocol reduced the phosphorylase (glycogenolytic) and phosphofructokinase (glycolytic) activities by 70%. The aerobic (citrate synthase activity) and fatty acid oxidative (3-hydroxyacyl-CoA dehydrogenase activity) capacities were not significantly increased by chronic stimulation and remained at about one-fourth those in the canine heart. The calcium-dependent sarcoplasmic reticulum adenosinetriphosphatase (ATPase) activity in the microsomal fraction, which was sixfold greater in the nonstimulated LDM than in the heart, was reduced by electrical stimulation to a level similar to that of the dog heart. The contractile capacity was evaluated by determining the percentage of types I and II fibers, the myofibrillar ATPase activity, and the proportion of myosin isoforms. The transformed muscle was comprised of 93 +/- 2% type I fibers, a myofibrillar ATPase activity similar to that in heart with primarily a slow-twitch muscle myosin isoform. In conclusion, electrical stimulation of canine LDM at 2 Hz for 6-8 wk resulted in two of the three biochemical systems, which confer physiological expression and fatigue resistance to muscle being transformed to resemble those of the myocardium.


This article has been cited by other articles:


Home page
 J. Thorac. Cardiovasc. Surg.Home page
N. Hedayati, J. T. Sherwood, S. J. Schomisch, J. L. Carino, and B. L. Cmolik
Circulatory benefits of diastolic counterpulsation in an ischemic heart failure model after aortomyoplasty
J. Thorac. Cardiovasc. Surg., June 1, 2002; 123(6): 1067 - 1073.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
B. L. Cmolik, D. R. Thompson, J. T. Sherwood, A. S. Geha, and D. T. George
Increased coronary artery blood flow with aortomyoplasty in chronic heart failure
Ann. Thorac. Surg., January 1, 2001; 71(1): 284 - 289.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online