Journal of Applied Physiology, Vol 66, Issue 4 1706-1714, Copyright © 1989 by American Physiological Society

ARTICLES

Calcium channel currents in isolated smooth muscle cells from human bronchus

R. Marthan, C. Martin, T. Amedee and J. Mironneau
Laboratoire de Physiologie Cellulaire, Institut de Biochimie et Neurochimie du Centre National de la Recherche Scientifique, Bordeaux, France.

An electrophysiological study was carried out on smooth muscle cells that were enzymatically dissociated from bundles of muscle fibers dissected out of human bronchi obtained at thoracotomy. These cells that retain the contractile properties of intact bundles were voltage-clamped by means of the whole-cell patch-clamp technique. Upon voltage steps from a holding potential of -60 mV to more positive levels, the initial inward current was followed by large outward currents that inactivated slowly. These were subsequently reduced by substituting Cs+ for K+ in the internal solution and by using Ba2+ instead of Ca2+ as a charge carrier in the external solution. Under these conditions, the inward current did not completely inactivate in the course of 300-ms voltage steps. Inward current measured after leak subtraction was activated at a membrane potential of -25.8 +/- 5 mV, was maximum at +18 +/- 4 mV, and had an apparent reversal potential of +52.5 +/- 5.5 mV (n = 5). The potential at which steady-state inactivation was half-maximum was -28 mV (n = 5). This inward current was identified as a calcium current on the following basis: 1) it was not altered by 10 microM tetrodotoxin (TTX) or by lowering to 10 mM external Na+ concentration; 2) it was blocked by 2.5 mM Co2+ or 1 microM PN 200-110; 3) it was enhanced by 1 microM BAY K 8644, which in addition suppressed the PN 200-110 blockade.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				K. Mizuta, G. Gallos, D. Zhu, F. Mizuta, F. Goubaeva, D. Xu, R. A. Panettieri Jr., J. Yang, and C. W. Emala Sr. Expression and coupling of neurokinin receptor subtypes to inositol phosphate and calcium signaling pathways in human airway smooth muscle cells Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L523 - L534. [Abstract] [Full Text] [PDF]

				S. Hirota, P. Helli, and L. J. Janssen Ionic mechanisms and Ca2+ handling in airway smooth muscle Eur. Respir. J., July 1, 2007; 30(1): 114 - 133. [Abstract] [Full Text] [PDF]

				C. Liu, J. Zuo, E. Pertens, P. B. Helli, and L. J. Janssen Regulation of Rho/ROCK signaling in airway smooth muscle by membrane potential and [Ca2+]i Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L574 - L582. [Abstract] [Full Text] [PDF]

				R. Marthan Store-operated calcium entry and intracellular calcium release channels in airway smooth muscle Am J Physiol Lung Cell Mol Physiol, May 1, 2004; 286(5): L907 - L908. [Full Text] [PDF]

				B. Ay, Y. S. Prakash, C. M. Pabelick, and G. C. Sieck Store-operated Ca2+ entry in porcine airway smooth muscle Am J Physiol Lung Cell Mol Physiol, May 1, 2004; 286(5): L909 - L917. [Abstract] [Full Text] [PDF]

				L. J. Janssen Ionic mechanisms and Ca2+ regulation in airway smooth muscle contraction: do the data contradict dogma? Am J Physiol Lung Cell Mol Physiol, June 1, 2002; 282(6): L1161 - L1178. [Abstract] [Full Text] [PDF]

				P. Berger, J. M. Tunon-De-Lara, J.-P. Savineau, and R. Marthan Signal Transduction in Smooth Muscle: Selected Contribution: Tryptase-induced PAR-2-mediated Ca2+ signaling in human airway smooth muscle cells J Appl Physiol, August 1, 2001; 91(2): 995 - 1003. [Abstract] [Full Text] [PDF]

				J.-M. Hyvelin, J.-P. Savineau, and R. Marthan Plasticity in Skeletal, Cardiac, and Smooth Muscle: Selected Contribution: Effect of the aldehyde acrolein on acetylcholine-induced membrane current in airway smooth muscle cells J Appl Physiol, February 1, 2001; 90(2): 750 - 754. [Abstract] [Full Text] [PDF]

				M. R. ACCOMAZZO, G. E. ROVATI, T. VIGANÒ, A. HERNANDEZ, A. BONAZZI, M. BOLLA, F. FUMAGALLI, S. VIAPPIANI, E. GALBIATI, S. RAVASI, et al. Leukotriene D4-Induced Activation of Smooth-Muscle Cells From Human Bronchi Is Partly Ca2+-Independent Am. J. Respir. Crit. Care Med., January 1, 2001; 163(1): 266 - 272. [Abstract] [Full Text]

				L. Tao, Y. Huang, and J.-P. Bourreau Control of the mode of excitation-contraction coupling by Ca2+ stores in bovine trachealis muscle Am J Physiol Lung Cell Mol Physiol, October 1, 2000; 279(4): L722 - L732. [Abstract] [Full Text] [PDF]

				J.-M. HYVELIN, C. MARTIN, E. ROUX, R. MARTHAN, and J.-P. SAVINEAU Human Isolated Bronchial Smooth Muscle Contains Functional Ryanodine/Caffeine-sensitive Ca-Release Channels Am. J. Respir. Crit. Care Med., August 1, 2000; 162(2): 687 - 694. [Abstract] [Full Text] [PDF]

				H. KURIYAMA, K. KITAMURA, T. ITOH, and R. INOUE Physiological Features of Visceral Smooth Muscle Cells, With Special Reference to Receptors and Ion Channels Physiol Rev, July 1, 1998; 78(3): 811 - 920. [Abstract] [Full Text] [PDF]

				I. Gorenne, C. Labat, J.-P. Gascard, X. Norel, N. Nashashibi, and C. Brink Leukotriene D4 Contractions in Human Airways are Blocked by SK&F 96365, an Inhibitor of Receptor-Mediated Calcium Entry J. Pharmacol. Exp. Ther., February 1, 1998; 284(2): 549 - 552. [Abstract] [Full Text]