| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Applied Physiology, Vol 66, Issue 3 1035-1039, Copyright © 1989 by American Physiological Society
ARTICLES |
J. Tamaoki, M. Kondo and T. Takizawa
Pulmonary Division, First Department of Medicine, Tokyo Women's Medical College, Japan.
To study the effect of adenosine 3',5'-cyclic monophosphate (cAMP) on respiratory ciliary activity, we measured ciliary beat frequency (CBF) of rabbit tracheal epithelium by a photoelectric method in response to cAMP analogues and agents that can increase endogenous cAMP production. Addition of 8-bromo-cAMP dose dependently enhanced CBF, with the maximal increase and the concentration necessary to produce a half-maximal response (KD) being 31.0 +/- 3.4% (SE) (P less than 0.001) and 3.2 +/- 1.5 x 10(-7) M, respectively. Other structurally dissimilar cAMP analogues dibutyryl cAMP and chlorophenylthio-cAMP likewise caused increases in CBF. The phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine and the adenylate cyclase stimulator forskolin also augmented CBF in a dose-dependent fashion and were accompanied by the increases in intracellular concentrations of cAMP. Ciliary discoordination was not observed in any of the experiments. These results suggest that cAMP may accelerate mucociliary clearance through the activation of ciliary motility and that intracellular cAMP levels appear to be an important determinant for the lung mucociliary transport functions.
This article has been cited by other articles:
|
|
 |
|
  A. Schmid, G. Bai, N. Schmid, M. Zaccolo, L. E. Ostrowski, G. E. Conner, N. Fregien, and M. Salathe Real-time analysis of cAMP-mediated regulation of ciliary motility in single primary human airway epithelial cells J. Cell Sci., October 15, 2006; 119(20): 4176 - 4186. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Wyatt, M. A. Forget, J. M. Adams, and J. H. Sisson Both cAMP and cGMP are required for maximal ciliary beat stimulation in a cell-free model of bovine ciliary axonemes Am J Physiol Lung Cell Mol Physiol, March 1, 2005; 288(3): L546 - L551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Gertsberg, V. Hellman, M. Fainshtein, S. Weil, S. D. Silberberg, M. Danilenko, and Z. Priel Intracellular Ca2+ Regulates the Phosphorylation and the Dephosphorylation of Ciliary Proteins Via the NO Pathway J. Gen. Physiol., October 25, 2004; 124(5): 527 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Shiima-Kinoshita, K.-Y. Min, T. Hanafusa, H. Mori, and T. Nakahari {beta}2-adrenergic regulation of ciliary beat frequency in rat bronchiolar epithelium: potentiation by isosmotic cell shrinkage J. Physiol., January 15, 2004; 554(2): 403 - 416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Wyatt, M. A. Forget, and J. H. Sisson Ethanol Stimulates Ciliary Beating by Dual Cyclic Nucleotide Kinase Activation in Bovine Bronchial Epithelial Cells Am. J. Pathol., September 1, 2003; 163(3): 1157 - 1166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. Kultgen, S. K. Byrd, L. E. Ostrowski, and S. L. Milgram Characterization of an A-Kinase Anchoring Protein in Human Ciliary Axonemes Mol. Biol. Cell, December 1, 2002; 13(12): 4156 - 4166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Ma, S. D. Silberberg, and Z. Priel Distinct Axonemal Processes Underlie Spontaneous and Stimulated Airway Ciliary Activity J. Gen. Physiol., November 25, 2002; 120(6): 875 - 885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. I. Frohock, C. Wijkstrom-Frei, and M. Salathe Effects of albuterol enantiomers on ciliary beat frequency in ovine tracheal epithelial cells J Appl Physiol, June 1, 2002; 92(6): 2396 - 2402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Zagoory, A. Braiman, and Z. Priel The Mechanism of Ciliary Stimulation by Acetylcholine: Roles of Calcium, PKA, and PKG J. Gen. Physiol., April 2, 2002; 119(4): 329 - 339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Morales, N. Barrera, P. Uribe, C. Mora, and M. Villalon Functional cross talk after activation of P2 and P1 receptors in oviductal ciliated cells Am J Physiol Cell Physiol, September 1, 2000; 279(3): C658 - C669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Wright, J. Seybold, A. Robichaud, I. M. Adcock, and P. J. Barnes Phosphodiesterase expression in human epithelial cells Am J Physiol Lung Cell Mol Physiol, October 1, 1998; 275(4): L694 - L700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Wyatt, J. R. Spurzem, K. May, and J. H. Sisson Regulation of ciliary beat frequency by both PKA and PKG in bovine airway epithelial cells Am J Physiol Lung Cell Mol Physiol, October 1, 1998; 275(4): L827 - L835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Tamaoki, M. Kondo, S. Takeuchi, H. Takemura, and A. Nagai Vasopressin Stimulates Ciliary Motility of Rabbit Tracheal Epithelium: Role of V1b Receptor-mediated Ca2+ Mobilization Am. J. Respir. Cell Mol. Biol., August 1, 1998; 19(2): 293 - 299. [Abstract] [Full Text]
|
|
|
|
|
|
S. L. Winters and D. B. Yeates Interaction between ion transporters and the mucociliary transport system in dog and baboon J Appl Physiol, October 1, 1997; 83(4): 1348 - 1359. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Tamaoki, A. Chiyotani, H. Takemura, K. Konno, T. Matsumoto, and Y. Ashida J. Pharmacol. Exp. Ther., June 1, 1997; 281(3): 1186 - 1190. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. J. TORPHY Phosphodiesterase Isozymes . Molecular Targets for Novel Antiasthma Agents Am. J. Respir. Crit. Care Med., February 1, 1997; 157(2): 351 - 370. [Full Text]
|
|
|
|
|
|
M Hansen, S Boitano, E. Dirksen, and M. Sanderson A role for phospholipase C activity but not ryanodine receptors in the initiation and propagation of intercellular calcium waves J. Cell Sci., January 7, 1995; 108(7): 2583 - 2590. [Abstract] [PDF]
|
|
|
|
|
|
M Salathe, M. Pratt, and A Wanner Protein kinase C-dependent phosphorylation of a ciliary membrane protein and inhibition of ciliary beating J. Cell Sci., January 12, 1993; 106(4): 1211 - 1220. [Abstract] [PDF]
|
|
|
|
|
|
O. Zagoory, A. Braiman, and Z. Priel The Mechanism of Ciliary Stimulation by Acetylcholine: Roles of Calcium, PKA, and PKG J. Gen. Physiol., April 2, 2002; 119(4): 329 - 339. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
