Journal of Applied Physiology AJP: Regulatory, Integrative and Comparative Physiology
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J Appl Physiol 65: 2513-2517, 1988;
8750-7587/88 $5.00
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Journal of Applied Physiology, Vol 65, Issue 6 2513-2517, Copyright © 1988 by American Physiological Society

ARTICLES

CO binding to hemoglobin and myoglobin in equilibrium with a gas phase of low PO2 value

A. Agostoni, M. Perrella, L. Sabbioneda and U. Zoni
Clinica Medica (V), University of Milan, Italy.

The aim of this paper was to measure the binding of CO to myoglobin and hemoglobin at various PO2 values. For this purpose we have studied an "in vitro" system made up of solutions of hemoglobin and myoglobin equilibrated in two connected tonometers with the same gas phase of various PO2 and PCO. The results indicate that a significant proportion of CO is released by hemoglobin and binds myoglobin at low PO2 values (approximately 2-3 Torr), in qualitative agreement with the predictions of a previous computer simulation of the "in vivo" system.


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