Journal of Applied Physiology
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J Appl Physiol 64: 2692-2695, 1988;
8750-7587/88 $5.00
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Journal of Applied Physiology, Vol 64, Issue 6 2692-2695, Copyright © 1988 by American Physiological Society

ARTICLES

In situ NADH laser fluorimetry of rat fast- and slow-twitch muscles during tetanus

D. Duboc, M. Muffat-Joly, G. Renault, M. Degeorges, M. Toussaint and J. J. Pocidalo
Institut National de la Sante et de la Recherche Medicale, Unite 13, Hopital Claude Bernard, Paris, France.

To investigate the variations of oxidation-reduction status of fast- and slow-twitch muscles during intense contractions, we performed in situ NADH laser fluorimetry during 25-s tetanus in extensor digitorum longus (EDL) and in soleus (SOL) muscles of eight Sprague-Dawley rats anesthetized with pentobarbital sodium. At base line the compensated NADH fluorescence (F0) was not significantly different between EDL and SOL. In EDL, tetanic stimulation induced an increase of F0, which rapidly reached a plateau that was 124% over the base-line value and stable until the end of the stimulation. In SOL, after an initial shouldering there was a continuous increase of F0 until the end of tetanus, reaching 275% of the base-line value. After the stimulation the initial rate of recovery was significantly faster in SOL than in EDL. We conclude that during and after intense contraction the variation of NADH content vs. time can be evaluated by in situ NADH laser fluorimetry in different muscle types. This nondestructive method can be helpful to differentiate in situ the various physiological or pathological oxidative capabilities of skeletal muscles.




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