Journal of Applied Physiology, Vol 64, Issue 6 2468-2473, Copyright © 1988 by American Physiological Society
Indomethacin, but not dazoxiben, reduced lung fluid filtration after E. coli infusion
R. Winn, B. Enderson, S. Price and C. L. Rice
Department of Physiology-Biophysics, University of Washington, Seattle 98195.
Goats were divided into three groups and given infusions of live
Escherichia coli bacteria. Group I received no treatment, group II was
treated with indomethacin (a cyclooxygenase inhibitor), and group III with
dazoxiben (a thromboxane synthase inhibitor). Double indicator-dilution
extravascular lung water (EVLW) in group I was significantly different from
the treated groups. There was an early increase in EVLW in group I and
group III but not in group II animals. At 6 h EVLW's in group I, group II,
and group III were 100, 45, and 30% above base line, respectively. Lymph
flow (QL) and lymph-to-plasma protein ratio (L/P) was not statistically
different between groups. Estimated total fluid filtration [QL +
d(EVLW)/dt] in group I and III was markedly elevated between 0 and 1.5-2 h
after E. coli infusion. Cardiac output (QT) decreased to 40% of base line
in group I, and it decreased slightly in group II because of the
indomethacin but did not decrease after E. coli. QT decreased in group III
but recovered more rapidly than group I. Mean pulmonary arterial pressure
increased more rapidly in group I and reached a higher peak than either
treated group. At 6 h these groups had similar pulmonary arterial and
pulmonary arterial wedge pressures. We conclude that 1) indomethacin but
not dazoxiben blocks the early increase in total fluid filtration after
bacterial infusion, 2) dazoxiben does not prevent the increased endothelial
permeability resulting from infusion of live bacteria, and 3) indomethacin
may somewhat ameliorate the endothelial permeability change.(ABSTRACT
TRUNCATED AT 250 WORDS)
