Journal of Applied Physiology, Vol 64, Issue 5 2245-2248, Copyright © 1988 by American Physiological Society

ARTICLES

Impaired muscle glycogen storage after muscle biopsy

D. L. Costill, D. R. Pearson and W. J. Fink
Human Performance Laboratory, Ball State University, Muncie, Indiana 47306.

To assess the effects of repeated needle biopsies on the rate of muscle glycogen repletion, eight male subjects were studied immediately after and 2 days after an exhaustive cycling bout. A single biopsy was obtained from the right vastus lateralis muscle immediately after an exhaustive cycling bout. Two days later, a sample was taken 1 cm lateral or medial to sample A. In four of these subjects, additional biopsies were taken 3 cm distal and proximal. A control specimen was also taken from the left leg 2 days after the exercise. Ten days after the exercise, muscle was again sampled from each leg of these four subjects. Analysis of these samples revealed that the initial biopsy impaired glycogen storage in the muscle taken 1 cm medial or lateral to the previous site. This reduction in glycogen storage was most pronounced in the first 2 days after the exercise. Samples taken distal and proximal to the initial biopsy contained, on the average, less glycogen than the contralateral leg, but these differences were only significantly different in the distal muscle sample. Alteration in muscle glycogen storage was seen to persist for 10 days after the first biopsy, suggesting that care must be taken in selecting the site for repeated biopsies from the same muscle.

This article has been cited by other articles:

				T. P. Gavin, C. B. Robinson, R. C. Yeager, J. A. England, L. W. Nifong, and R. C. Hickner Angiogenic growth factor response to acute systemic exercise in human skeletal muscle J Appl Physiol, January 1, 2004; 96(1): 19 - 24. [Abstract] [Full Text] [PDF]

				H. W. Goforth Jr., D. Laurent, W. K. Prusaczyk, K. E. Schneider, K. F. Petersen, and G. I. Shulman Effects of depletion exercise and light training on muscle glycogen supercompensation in men Am J Physiol Endocrinol Metab, December 1, 2003; 285(6): E1304 - E1311. [Abstract] [Full Text] [PDF]

				L. M. Burke, D. J. Angus, G. R. Cox, N. K. Cummings, M. A. Febbraio, K. Gawthorn, J. A. Hawley, M. Minehan, D. T. Martin, and M. Hargreaves Effect of fat adaptation and carbohydrate restoration on metabolism and performance during prolonged cycling J Appl Physiol, December 1, 2000; 89(6): 2413 - 2421. [Abstract] [Full Text] [PDF]

				J. A. Carrithers, D. L. Williamson, P. M. Gallagher, M. P. Godard, K. E. Schulze, and S. W. Trappe Effects of postexercise carbohydrate-protein feedings on muscle glycogen restoration J Appl Physiol, June 1, 2000; 88(6): 1976 - 1982. [Abstract] [Full Text] [PDF]

				S. Asp, J. R. Daugaard, T. Rohde, K. Adamo, and T. Graham Muscle glycogen accumulation after a marathon: roles of fiber type and pro- and macroglycogen J Appl Physiol, February 1, 1999; 86(2): 474 - 478. [Abstract] [Full Text] [PDF]

				J. B. Mitchell, F. X. Pizza, A. Paquet, B. J. Davis, M. B. Forrest, and W. A. Braun Influence of carbohydrate status on immune responses before and after endurance exercise J Appl Physiol, June 1, 1998; 84(6): 1917 - 1925. [Abstract] [Full Text] [PDF]

				S. Asp, T. Rohde, and E. A. Richter Impaired muscle glycogen resynthesis after a marathon is not caused by decreased muscle GLUT-4 content J Appl Physiol, November 1, 1997; 83(5): 1482 - 1485. [Abstract] [Full Text] [PDF]

				R. C. Hickner, J. S. Fisher, P. A. Hansen, S. B. Racette, C. M. Mier, M. J. Turner, and J. O. Holloszy Muscle glycogen accumulation after endurance exercise in trained and untrained individuals J Appl Physiol, September 1, 1997; 83(3): 897 - 903. [Abstract] [Full Text] [PDF]