| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Applied Physiology, Vol 64, Issue 5 2092-2099, Copyright © 1988 by American Physiological Society
ARTICLES |
E. R. Pacht and W. B. Davis
Department of Medicine, Ohio State University 43210.
Lung epithelial lining fluid (ELF) is a thin layer of plasma ultrafiltrate and locally secreted substances that may provide antioxidant protection and serve as a "front-line" defense for the lower respiratory tract epithelium. To characterize the antioxidant properties of ELF, young, healthy, nonsmoking volunteers underwent bronchoalveolar lavage with determination of ELF volumes and ELF proteins. ELF (greater than 0.4 ml) is a potent inhibitor of lipid peroxidation as measured by malondialdehyde (MDA) production in an in vitro iron-dependent assay system. Two serum proteins, transferrin and ceruloplasmin, were quantitated in ELF and found to be potent inhibitors of lipid peroxidation. Other ELF components, including vitamin E, vitamin C, and albumin, did not function as antioxidants in this system. Several experimental observations suggest that ELF transferrin was more important than ceruloplasmin in inhibiting lipid peroxidation: 1) ELF concentrations of transferrin were 20-fold higher than those for ceruloplasmin; 2) ELF antioxidant activity was abolished by preincubation with Fe3+; 3) ELF antioxidant activity was minimally affected by sodium azide, which is known to inhibit ceruloplasmin ferroxidase activity; and 4) ELF ceruloplasmin ferroxidase activity was virtually nondetectable. ELF possesses a significant antioxidant activity that may be important in vivo in protecting the lung from oxidant injury.
This article has been cited by other articles:
|
|
 |
|
  E. A. Heilig, K. J. Thompson, R. M. Molina, A. R. Ivanov, J. D. Brain, and M. Wessling-Resnick Manganese and iron transport across pulmonary epithelium Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1247 - L1259. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chen, Z. Chen, N. R. Chintagari, M. Bhaskaran, N. Jin, T. Narasaraju, and L. Liu Alveolar type I cells protect rat lung epithelium from oxidative injury J. Physiol., May 1, 2006; 572(3): 625 - 638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H Kanazawa, S Shiraishi, K Hirata, and J Yoshikawa Imbalance between levels of nitrogen oxides and peroxynitrite inhibitory activity in chronic obstructive pulmonary disease Thorax, February 1, 2003; 58(2): 106 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Sun, K. Crissman, J. Norwood, J. Richards, R. Slade, and G. E. Hatch Oxidative interactions of synthetic lung epithelial lining fluid with metal-containing particulate matter Am J Physiol Lung Cell Mol Physiol, October 1, 2001; 281(4): L807 - L815. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-Y. Xu, J.-S. Yang, H. Link, and B.-G. Xiao SIN-1, a Nitric Oxide Donor, Ameliorates Experimental Allergic Encephalomyelitis in Lewis Rats in the Incipient Phase: The Importance of the Time Window J. Immunol., May 1, 2001; 166(9): 5810 - 5816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Ghio, J. D. Carter, J. H. Richards, K. M. Crissman, H. H. Bobb, and F. Yang Diminished injury in hypotransferrinemic mice after exposure to a metal-rich particle Am J Physiol Lung Cell Mol Physiol, May 1, 2000; 278(5): L1051 - L1061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Yang, J. J. Coalson, H. H. Bobb, J. D. Carter, J. Banu, and A. J. Ghio Resistance of hypotransferrinemic mice to hyperoxia-induced lung injury Am J Physiol Lung Cell Mol Physiol, December 1, 1999; 277(6): L1214 - L1223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Freed, R. Cueto, and W. A. Pryor Antioxidant transport modulates peripheral airway reactivity and inflammation during ozone exposure J Appl Physiol, November 1, 1999; 87(5): 1595 - 1603. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Balagopalakrishna, L. Paka, S. Pillarisetti, and I. J. Goldberg Lipolysis-induced iron release from diferric transferrin: possible role of lipoprotein lipase in LDL oxidation J. Lipid Res., July 1, 1999; 40(7): 1347 - 1356. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. C. Webb and A. W. Cripps Immunization with Recombinant Transferrin Binding Protein B Enhances Clearance of Nontypeable Haemophilus influenzae from the Rat Lung Infect. Immun., May 1, 1999; 67(5): 2138 - 2144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. KANAZAWA, S. SHIRAISHI, T. OKAMOTO, K. HIRATA, and J. YOSHIKAWA Inhibition of Bronchoprotective Effects of beta 2-Adrenoceptor Agonists by Peroxynitrite in Guinea Pig Airways Am. J. Respir. Crit. Care Med., April 1, 1999; 159(4): 1272 - 1276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kennedy, A. J. Ghio, W. Reed, J. Samet, J. Zagorski, J. Quay, J. Carter, L. Dailey, J. R. Hoidal, and R. B. Devlin Copper-dependent Inflammation and Nuclear Factor-kappa B Activation by Particulate Air Pollution Am. J. Respir. Cell Mol. Biol., September 1, 1998; 19(3): 366 - 378. [Abstract] [Full Text]
|
|
|
|
|
|
F. Mateos, J. H Brock, and J. L. Pérez-Arellano Iron metabolism in the lower respiratory tract Thorax, July 1, 1998; 53(7): 594 - 600. [Full Text]
|
|
|
|
|
|
A. J. Ghio, J. D. Carter, J. H. Richards, L. E. Brighton, J. C. Lay, and R. B. Devlin Disruption of normal iron homeostasis after bronchial instillation of an iron-containing particle Am J Physiol Lung Cell Mol Physiol, March 1, 1998; 274(3): L396 - L403. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
