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J Appl Physiol 64: 936-943, 1988;
8750-7587/88 $5.00
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Journal of Applied Physiology, Vol 64, Issue 3 936-943, Copyright © 1988 by American Physiological Society

ARTICLES

Effects of cyclo- and lipoxygenase inhibitors on hypoxic vasoconstriction in isolated ferret lungs

J. E. Gottlieb, M. McGeady, N. F. Adkinson Jr and J. T. Sylvester
Department of Medicine, Johns Hopkins Medical Institutions, Francis Scott Key Medical Center, Baltimore, Maryland 21224.

To evaluate the role of leukotrienes in hypoxic pulmonary vasoconstriction, we measured steady-state pressor responses to graded hypoxia in isolated ferret lungs perfused with autologous blood containing 0.001, 0.03, 1, or 3 mM nordihydroguaiaretic acid (NDGA), 1 mM BW 755C, or 0.02-0.05 mM indomethacin. Untreated lungs served as controls. Perfusate concentrations of thromboxane B2 and 6-ketoprostaglandin F1 alpha, measured by radioimmunoassay, were markedly reduced in all treated lungs, indicating inhibition of cyclooxygenase. The maximum pressor response to hypoxia measured at a blood flow of 50 ml.min-1. kg-1 averaged 26.6 +/- 2.4 Torr in untreated lungs and was not affected by BW 755C or 0.001-0.03 mM NDGA. Because BW 755C and NDGA inhibited cyclooxygenase at concentrations that did not affect hypoxic vasoconstriction and because both agents are thought to inhibit lipoxygenase with a potency greater than or equal to that with which they inhibit cyclooxygenase, these results do not support the possibility that hypoxic pulmonary vasoconstriction was mediated by leukotrienes. At concentrations of 1 and 3 mM, NDGA inhibited the maximum hypoxic pressor response by 57 and 95%, respectively. The mechanism of this attenuation is unknown; however, it was apparently not due to cyclooxygenase inhibition, since indomethacin enhanced the maximum hypoxic pressor response by 45%. Nor was it due to blockade of calcium entry or interference with the contractile process in pulmonary vascular smooth muscle, since 1 mM NDGA did not inhibit vasoconstrictor responses to KCl or prostaglandin F2 alpha.


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Q. Liu, J. S. K. Sham, L. A. Shimoda, and J. T. Sylvester
Hypoxic constriction of porcine distal pulmonary arteries: endothelium and endothelin dependence
Am J Physiol Lung Cell Mol Physiol, May 1, 2001; 280(5): L856 - L865.
[Abstract] [Full Text] [PDF]


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