Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 63: 1558-1566, 1987;
8750-7587/87 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by De Jongste, J. C.
Right arrow Articles by Kerrebijn, K. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by De Jongste, J. C.
Right arrow Articles by Kerrebijn, K. F.

Journal of Applied Physiology, Vol 63, Issue 4 1558-1566, Copyright © 1987 by American Physiological Society

ARTICLES

Nonneural components in the response of fresh human airways to electric field stimulation

J. C. De Jongste, H. Mons, I. L. Bonta and K. F. Kerrebijn
Department of Pediatric Respiratory Diseases, Erasmus University, Rotterdam, The Netherlands.

Fresh human bronchi, obtained at thoracotomy and maintained at 37 degrees C, were studied in vitro to investigate their response to electric field stimulation (EFS). We found complex responses that were not only composed of a rapid initial nerve-mediated cholinergic contraction and a non-adrenergic nerve-mediated relaxation, but, in 80% of preparations, also of a tonic contraction with a sustained time course. This sustained phase was not blocked by the nervous conductance blocker tetrodotoxin (TTX) and was therefore not neurally mediated. Controlled transient cooling to 4 degrees C in the organ bath reduced this sustained phase selectively for several hours. The leukotriene (LT) antagonist FPL 55712, dexamethasone, which inhibits phospholipase A2, and the antiasthmatic drug cromolyn all reduced the sustained phase significantly. In 20% of strips, an additional TTX-resistant contraction was seen directly after the cholinergic phase. This contraction could be inhibited by indomethacin. A similar small peak sometimes appeared after selective blocking of either the cholinergic or the sustained phases. Experiments in which the epithelium was removed from the strips suggested that this indomethacin-sensitive response, but not the sustained phase, was dependent on the presence of epithelium. These results show that EFS of fresh human bronchi stimulated cholinergic and nonadrenergic inhibitory nerves and gave rise to a partly epithelium-dependent synthesis of arachidonic acid metabolites, which caused contractile responses that interfered with the neurally mediated responses.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. Wang, V. Pozzato, G. Turato, A. Madamanchi, T. M. Murphy, and P. Chitano
Reduced spontaneous relaxation in immature guinea pig airway smooth muscle is associated with increased prostanoid release
Am J Physiol Lung Cell Mol Physiol, May 1, 2008; 294(5): L964 - L973.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
F.-X. Blanc, S. Salmeron, C. Coirault, M. Bard, E. Fadel, E. Dulmet, P. Dartevelle, and Y. Lecarpentier
Effects of load and tone on the mechanics of isolated human bronchial smooth muscle
J Appl Physiol, February 1, 1999; 86(2): 488 - 495.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. Chitano, C. M. Cox, and T. M. Murphy
Relaxation of guinea pig trachealis during electrical field stimulation increases with age
J Appl Physiol, May 1, 2002; 92(5): 1835 - 1842.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online