Journal of Applied Physiology, Vol 63, Issue 1 359-367, Copyright © 1987 by American Physiological Society

ARTICLES

Liposome-mediated augmentation of catalase in alveolar type II cells protects against H2O2 injury

B. J. Buckley, A. K. Tanswell and B. A. Freeman

Oxidant injury to the alveolar epithelium can be mediated by exposure to oxidant gases such as O2 at high concentrations and O3, inflammatory cell-derived reactive O2 species, and the intracellular metabolism of xenobiotics such as paraquat. An in vitro model of alveolar epithelial oxidant injury was developed based on exposure of cultured rat type II pneumocytes to superoxide and hydrogen peroxide (H2O2) enzymatically generated in the culture medium. Cytotoxicity was assessed by the release of lactate dehydrogenase (LDH) into the culture medium, which was a more reliable indicator of damage than release of 51Cr by prelabeled cells. Incubation of cells for 6-8 h with xanthine plus xanthine oxidase and glucose plus glucose oxidase induced the release of greater than 50% of total intracellular LDH. Oxidant exposure also resulted in significant detachment of cells from culture dishes. Modulation of oxidant damage was accomplished using liposomes as vectors for the delivery of catalase. Treatment of cells with catalase liposomes for 2 h resulted in augmentation of cellular catalase specific activities up to 631% of controls. Catalase was partitioned into intracellular and surface-associated compartments in catalase liposome-treated cells. Partial and complete protection against oxidant injury, induced by xanthine plus xanthine oxidase and glucose plus glucose oxidase, respectively, was achieved by pretreatment of cells with catalase liposomes. LDH release during oxidant exposure was inversely related to augmentation of cellular catalase activities. Catalase liposome-treated cells also exhibited an enhanced ability to scavenge enzymatically generated H2O2 from the culture medium. These observations suggest a useful approach to modulation of alveolar injury induced by reactive O2 species.

This article has been cited by other articles:

				G Graziani, G D'Argenio, C Tuccillo, C Loguercio, A Ritieni, F Morisco, C Del Vecchio Blanco, V Fogliano, and M Romano Apple polyphenol extracts prevent damage to human gastric epithelial cells in vitro and to rat gastric mucosa in vivo Gut, February 1, 2005; 54(2): 193 - 200. [Abstract] [Full Text] [PDF]

				V. L. Kinnula and J. D. Crapo Superoxide Dismutases in the Lung and Human Lung Diseases Am. J. Respir. Crit. Care Med., June 15, 2003; 167(12): 1600 - 1619. [Abstract] [Full Text] [PDF]

				X. Luo, R. Belcastro, J. Cabacungan, V. Hannam, A. Negus, Y. Wen, J. Plumb, J. Hu, B. Steer, D. R. Koehler, et al. Transfection of lung cells in vitro and in vivo: effect of antioxidants and intraliposomal bFGF Am J Physiol Lung Cell Mol Physiol, May 1, 2003; 284(5): L817 - L825. [Abstract] [Full Text] [PDF]

				T. Nishiyama, R. J. Y. Ho, D. D. Shen, and T. L. Yaksh The Effects of Intrathecal Morphine Encapsulated in L- and D-Dipalmitoylphosphatidyl Choline Liposomes on Acute Nociception in Rats Anesth. Analg., August 1, 2000; 91(2): 423 - 428. [Abstract] [Full Text] [PDF]

				C. Rusznak, R. J. Sapsford, J. L. Devalia, R. Justin John, E. L. Hewitt, A. G. Lamont, A. J. Wood, S. S. Shah, R. J. Davies, and S. Lozewicz Cigarette Smoke Potentiates House Dust Mite Allergen-Induced Increase in the Permeability of Human Bronchial Epithelial Cells In Vitro Am. J. Respir. Cell Mol. Biol., June 1, 1999; 20(6): 1238 - 1250. [Abstract] [Full Text]

				A. K. Tanswell, O. Staub, R. Iles, R. Belcastro, J. Cabacungan, L. Sedlackova, B. Steer, Y. Wen, J. Hu, and H. O'Brodovich Liposome-mediated transfection of fetal lung epithelial cells: DNA degradation and enhanced superoxide toxicity Am J Physiol Lung Cell Mol Physiol, September 1, 1998; 275(3): L452 - L460. [Abstract] [Full Text] [PDF]