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J Appl Physiol 61: 1383-1387, 1986;
8750-7587/86 $5.00
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Journal of Applied Physiology, Vol 61, Issue 4 1383-1387, Copyright © 1986 by American Physiological Society

ARTICLES

Effect of perfusate hematocrit on urea permeability-surface area in isolated dog lung

R. E. Parker, R. J. Roselli, F. R. Haselton and T. R. Harris

Seven dog lower left lung lobes were statically inflated and perfused at a constant rate for each lobe with a perfusate in which the hematocrit was altered over a wide range. The permeability-surface area of urea was calculated from multiple indicator dilution curves using two separate injectates for each hematocrit level. One injectate contained only 125I-albumin as the vascular reference tracer and the other contained both 51Cr-erythrocytes and 125I-albumin as the vascular reference tracers; both contained [14C]urea as the permeating tracer. The results strongly indicate that the phenomenon of "erythrocyte trapping" of urea does not affect the calculation of urea permeability-surface area product provided the appropriate albumin-erythrocyte composite reference tracer is utilized in its calculation.




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