Journal of Applied Physiology, Vol 61, Issue 2 561-564, Copyright © 1986 by American Physiological Society
Liberation of muscle carbonic anhydrase into serum during extensive exercise
H. K. Vaananen, M. Leppilampi, J. Vuori and T. E. Takala
A sensitive radioimmunoassay method for measurement of human carbonic
anhydrase III (CA III), a specific enzyme of skeletal muscle, was
established. The effect of 24-h competitive running on serum CA III (S-CA
III) was studied in healthy male long-distance runners. S-CA III increased
gradually during the first 18 h 410-fold above the initial values. No
further increase was observed during the last 6 h. On a day after the
termination of the race S-CA III was not statistically different from the
initial value. The activity of serum creatine kinase (S-CK) and lactate
dehydrogenase (S-LDH) increased gradually through the run being elevated
also 1 or 2 days afterward. The lack of increase in S-CA III during the
last hours of exercise, and more rapid decrease afterward than in S-CK or
S-LDH, may reflect differences in the rates of penetration through the
sarcolemma and different degrees of injury in different fiber types or in
the half-lives of these enzymes in serum.
