Journal of Applied Physiology, Vol 59, Issue 3 928-934, Copyright © 1985 by American Physiological Society

ARTICLES

Long-term clearance of liquid and protein from the lungs of unanesthetized sheep

M. A. Matthay, Y. Berthiaume and N. C. Staub

We measured the removal of 100 ml of autologous serum from the air spaces and lungs of unanesthetized, spontaneously breathing sheep at 4, 12, and 24 h. In the first 4 h, there was a rapid clearance of the liquid volume (8.3%/h), similar to our results in anesthetized ventilated sheep (Matthay et al., J. Appl. Physiol. 53: 96-104, 1982). However, liquid removal progressively slowed to 3.3 and 1.4%/h at 12 and 24 h, respectively. In contrast, protein clearance (as measured by 125I-albumin instilled with the serum) was monoexponential and slow (1%/h). The slowing of liquid clearance appears to be a function of the rising protein osmotic pressure of the residual protein in the air spaces (protein concentration doubled in 24 h). Because protein solutions are chemotactic for neutrophils, we quantified the movement of liquid from the extracellular space into the alveolar compartment with a plasma protein tracer (131I-albumin), so that our final calculation of alveolar liquid clearance would take into account bidirectional movement of liquid across the alveolar barrier. The corrected values for net liquid clearance are slightly faster (less than 10% of the instilled volume).

This article has been cited by other articles:

				L. B. Ware Modeling human lung disease in animals Am J Physiol Lung Cell Mol Physiol, February 1, 2008; 294(2): L149 - L150. [Full Text] [PDF]

				S. U. Sixt, M. Beiderlinden, H. P. Jennissen, and J. Peters Extracellular proteasome in the human alveolar space: a new housekeeping enzyme? Am J Physiol Lung Cell Mol Physiol, May 1, 2007; 292(5): L1280 - L1288. [Abstract] [Full Text] [PDF]

				N. de Prost, D. Dreyfuss, and G. Saumon Evaluation of two-way protein fluxes across the alveolo-capillary membrane by scintigraphy in rats: effect of lung inflation J Appl Physiol, February 1, 2007; 102(2): 794 - 802. [Abstract] [Full Text] [PDF]

				M. A. Matthay, L. Robriquet, and X. Fang Alveolar Epithelium: Role in Lung Fluid Balance and Acute Lung Injury Proceedings of the ATS, October 1, 2005; 2(3): 206 - 213. [Abstract] [Full Text] [PDF]

				R. H. Hastings, H. G. Folkesson, and M. A. Matthay Mechanisms of alveolar protein clearance in the intact lung Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L679 - L689. [Abstract] [Full Text] [PDF]

				M. A. Matthay, C. Clerici, and G. Saumon Lung Edema Clearance: 20 Years of Progress: Invited Review: Active fluid clearance from the distal air spaces of the lung J Appl Physiol, October 1, 2002; 93(4): 1533 - 1541. [Abstract] [Full Text] [PDF]

				M. A. Matthay, H. G. Folkesson, and C. Clerici Lung Epithelial Fluid Transport and the Resolution of Pulmonary Edema Physiol Rev, July 1, 2002; 82(3): 569 - 600. [Abstract] [Full Text] [PDF]

				R. L. Conhaim, K. E. Watson, S. J. Lai-Fook, and B. A. Harms Transport properties of alveolar epithelium measured by molecular hetastarch absorption in isolated rat lungs J Appl Physiol, October 1, 2001; 91(4): 1730 - 1740. [Abstract] [Full Text] [PDF]

				L. B. WARE and M. A. MATTHAY Alveolar Fluid Clearance Is Impaired in the Majority of Patients with Acute Lung Injury and the Acute Respiratory Distress Syndrome Am. J. Respir. Crit. Care Med., May 1, 2001; 163(6): 1376 - 1383. [Abstract] [Full Text]

				J. A. Frank, Y. Wang, O. Osorio, and M. A. Matthay beta -Adrenergic agonist therapy accelerates the resolution of hydrostatic pulmonary edema in sheep and rats J Appl Physiol, October 1, 2000; 89(4): 1255 - 1265. [Abstract] [Full Text] [PDF]

				N. Fukuda, H. G. Folkesson, and M. A. Matthay Relationship of interstitial fluid volume to alveolar fluid clearance in mice: ventilated vs. in situ studies J Appl Physiol, August 1, 2000; 89(2): 672 - 679. [Abstract] [Full Text] [PDF]

				R. H. Hastings, J. T. Berg, D. Summers-Torres, D. W. Burton, and L. J. Deftos Parathyroid hormone-related protein reduces alveolar epithelial cell proliferation during lung injury in rats Am J Physiol Lung Cell Mol Physiol, July 1, 2000; 279(1): L194 - L200. [Abstract] [Full Text] [PDF]

				R. J. Kaner, J. V. Ladetto, R. Singh, N. Fukuda, M. A. Matthay, and R. G. Crystal Lung Overexpression of the Vascular Endothelial Growth Factor Gene Induces Pulmonary Edema Am. J. Respir. Cell Mol. Biol., June 1, 2000; 22(6): 657 - 664. [Abstract] [Full Text]

				W. G. OLIVERA, D. E. CICCOLELLA, N. BARQUIN, K. M. RIDGE, D. H. RUTSCHMAN, D. B. YEATES, and J. I. SZNAJDER Aldosterone Regulates Na,K-ATPase and Increases Lung Edema Clearance in Rats Am. J. Respir. Crit. Care Med., February 1, 2000; 161(2): 567 - 573. [Abstract] [Full Text]

				A. Lazrak, V. G. Nielsen, and S. Matalon Mechanisms of increased Na+ transport in ATII cells by cAMP: we agree to disagree and do more experiments Am J Physiol Lung Cell Mol Physiol, February 1, 2000; 278(2): L233 - L238. [Abstract] [Full Text] [PDF]

				C. HERMANS and A. BERNARD Lung Epithelium-specific Proteins . Characteristics and Potential Applications as Markers Am. J. Respir. Crit. Care Med., February 1, 1999; 159(2): 646 - 678. [Full Text]

				Y. Berthiaume, O. Lesur, and A. Dagenais Treatment of adult respiratory distress syndrome: plea for rescue therapy of the alveolar epithelium Thorax, February 1, 1999; 54(2): 150 - 160. [Full Text]

				A. R. Campbell, H. G. Folkesson, Y. Berthiaume, J. Gutkowska, S. Suzuki, and M. A. Matthay Alveolar epithelial fluid clearance persists in the presence of moderate left atrial hypertension in sheep J Appl Physiol, January 1, 1999; 86(1): 139 - 151. [Abstract] [Full Text] [PDF]

				Y. Guo, M. D. Duvall, J. P. Crow, and S. Matalon Nitric oxide inhibits Na+ absorption across cultured alveolar type II monolayers Am J Physiol Lung Cell Mol Physiol, March 1, 1998; 274(3): L369 - L377. [Abstract] [Full Text] [PDF]

				A. Norlin, N. Finley, P. Abedinpour, and H. G. Folkesson Alveolar liquid clearance in the anesthetized ventilated guinea pig Am J Physiol Lung Cell Mol Physiol, February 1, 1998; 274(2): L235 - L243. [Abstract] [Full Text] [PDF]

				H. G. Folkesson and M. A. Matthay Inhibition of CD18 or CD11b attenuates acute lung injury after acid instillation in rabbits J Appl Physiol, June 1, 1997; 82(6): 1743 - 1750. [Abstract] [Full Text] [PDF]

				M. D. Johnson, J. H. Widdicombe, L. Allen, P. Barbry, and L. G. Dobbs Alveolar epithelial type I cells contain transport proteins and transport sodium, supporting an active role for type I cells in regulation of lung liquid homeostasis PNAS, February 19, 2002; 99(4): 1966 - 1971. [Abstract] [Full Text] [PDF]