Journal of Applied Physiology, Vol 58, Issue 6 1949-1955, Copyright © 1985 by American Physiological Society
Albumin attenuation of oleic acid edema in dog lung depleted of blood components
W. F. Hofman and I. C. Ehrhart
Circulating fatty acids are normally transported principally bound to serum
albumin. We examined whether administering oleic acid (OA) in a
concentrated albumin solution would attenuate its edemogenic potential in
the isolated dog lung lobe perfused with a solution nearly depleted of
blood cellular and protein components. The isolated ventilated lower left
lobe (LLL) was perfused (7.3 +/- 0.6 ml X min-1 X g LLL-1) with a balanced
salt solution containing 6% dextran and approximately 10% serum (vol/vol).
Hourly weight gain, net LLL weight gain, and wet-to-dry weight ratio (W/D)
were used as indices of extravascular lung fluid changes. Group I lobes (n
= 5) were given saline, whereas both group II (n = 5) and III (n = 5) lobes
were administered 1 microliter OA/kg body wt. The OA was incubated with 5
ml of albumin solution containing approximately 640 mg of bovine fatty
acid-free albumin before infusion into group III lobes. Group I gained
weight at rate of 10.8 +/- 0.5 g X h-1 X 100 g LLL-1 after saline, whereas
group II exhibited a greater (P less than 0.005) rate of weight gain of 42
+/- 13 after OA. Group III weight gain of 8.4 +/- 0.5 g X h-1 X 100 g LLL-1
was not different (P greater than 0.05) from group I but was lower (P less
than 0.005) than group II.(ABSTRACT TRUNCATED AT 250 WORDS)
