Journal of Applied Physiology, Vol 56, Issue 4 862-867, Copyright © 1984 by American Physiological Society

ARTICLES

Filtration coefficient obtained by stepwise pressure elevation in isolated dog lung

I. C. Ehrhart, W. M. Granger and W. F. Hofman

The base-line capillary filtration coefficient (Kf) obtained from rates of lobe weight gain during stepwise vascular pressure elevation is reported to be threefold greater in isolated than in intact dog lung. To further evaluate the stepwise pressure elevation technique, we obtained Kf in control and oleic acid-injured isolated lung. The left lower lung lobe was removed, placed on a balance, ventilated, and pump perfused with autogenous blood. Saline (n = 6) or oleic acid (n = 6) was infused, and rate of lobe weight gain was obtained during stepwise pressure elevation. Kf averaged 0.071 +/- 0.012 and 0.243 +/- 0.027 ml X min-1 X Torr-1 X 100 g-1 in the control and injured lobes, respectively. Stepwise pressure elevation can yield a base-line Kf in isolated lung similar to Kf's obtained from this and other gravimetric methods in intact and isolated lung. Furthermore, Kf increased severalfold following lung injury with oleic acid. The stepwise pressure elevation technique for Kf determination in isolated lung can be a useful tool for quantitating changes in vascular permeability.

This article has been cited by other articles:

				R. M. Dongaonkar, G. A. Laine, R. H. Stewart, and C. M. Quick Balance point characterization of interstitial fluid volume regulation Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2009; 297(1): R6 - R16. [Abstract] [Full Text] [PDF]

				R. M. Dongaonkar, C. M. Quick, R. H. Stewart, R. E. Drake, C. S. Cox Jr., and G. A. Laine Edemagenic gain and interstitial fluid volume regulation Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2008; 294(2): R651 - R659. [Abstract] [Full Text] [PDF]

				A. S. Verkman, M. A. Matthay, and Y. Song Aquaporin water channels and lung physiology Am J Physiol Lung Cell Mol Physiol, May 1, 2000; 278(5): L867 - L879. [Abstract] [Full Text] [PDF]

				Y. Song, T. Ma, M. A. Matthay, and A.S. Verkman Role of Aquaporin-4 in Airspace-to-Capillary Water Permeability in Intact Mouse Lung Measured by a Novel Gravimetric Method J. Gen. Physiol., January 1, 2000; 115(1): 17 - 27. [Abstract] [Full Text] [PDF]

				H. Kishima, S.-i. Takeda, S. Miyoshi, A. Matsumura, M. Minami, T. Utsumi, K.-i. Omori, K. Nakahara, and H. Matsuda Microvascular Permeability of the Non-Heart-Beating Rabbit Lung After Warm Ischemia and Reperfusion: Role of Neutrophil Elastase Ann. Thorac. Surg., April 1, 1998; 65(4): 913 - 918. [Abstract] [Full Text] [PDF]

				J. W. Klaesner, N. A. Pou, R. E. Parker, C. Finney, and R. J. Roselli Optical measurement of isolated canine lung filtration coefficients at normal hematocrits J Appl Physiol, December 1, 1997; 83(6): 1976 - 1985. [Abstract] [Full Text] [PDF]

				J. E. Hall, W. F. Hofman, and I. C. Ehrhart Hemodynamic and Vascular Permeability Effects of Lipid Emulsion in the Isolated Canine Lung Lobe JPEN J Parenter Enteral Nutr, July 1, 1990; 14(4): 376 - 380. [Abstract] [PDF]