Journal of Applied Physiology
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J Appl Physiol 56: 1120-1125, 1984;
8750-7587/84 $5.00
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Journal of Applied Physiology, Vol 56, Issue 4 1120-1125, Copyright © 1984 by American Physiological Society

ARTICLES

Calibration methods in respiratory calorimetry

B. A. Young, T. W. Fenton and J. A. McLean

Methods for calibration of respiratory gas exchange measurement systems are evaluated. A new method is described in which O2 is removed from the subject chamber or gas stream and measured gravimetrically in a small combustion chamber containing steel wool. The "Fe-burner" method was tested by comparison with the N2-injection method and found to be of similar accuracy. The new method is easy to perform, quick, and readily applicable to both open- and closed-circuit respiratory systems. It has a rate of O2 uptake that can be adjusted to be similar to the rate by an experimental subject (10-600 kg). Complete calibration of open- and closed-circuit indirect calorimetric systems can be achieved when the Fe-burner method, involving measurement of O2 uptake, is combined with the gravimetric injection of specific gases (CO2, methane) produced by the subject.


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