Journal of Applied Physiology, Vol 55, Issue 4 1219-1224, Copyright © 1983 by American Physiological Society

ARTICLES

Effect of a 42.2-km footrace and subsequent rest or exercise on muscle glycogen and enzymes

W. M. Sherman, D. L. Costill, W. J. Fink, F. C. Hagerman, L. E. Armstrong and T. F. Murray

The purpose of this study was to determine the effect of running a marathon and subsequent rest or exercise during a 7-day recovery period on muscle glycogen, glycogen synthase, hexokinase, and the activity of hexose monophosphate pathway enzymes. Ten well-trained runners were randomly assigned to either a postmarathon rest or exercise group. The rest group (n = 5) performed no physical activity, whereas the exercise group ran at their highest tolerable volitional intensity for 20-45 min during the recovery period. Muscle biopsies were obtained from the gastrocnemius before and after the marathon and 1, 3, 5, and 7 days after the marathon. Muscle glycogen was supercompensated before the marathon (196 mmol glucosyl units kg wet tissue wt-1, mmol X kg-1) and was reduced to 25 mmol X kg-1 after the marathon. Seven days later it was 141 and 109 mmol X kg-1 in the rest and exercise groups, respectively. Measurement of glycogen synthase activity ratio indicated an active enzyme only immediately postmarathon, whereas measurement of glycogen synthase fractional velocity and relative velocity indicated an active enzyme immediately postmarathon and 1 day postmarathon. Hexokinase activity was significantly elevated immediately after the marathon and remained elevated through 5 days postmarathon. The activities of the hexose monophosphate pathway enzymes declined steadily during the experimental period and were significantly reduced 5 and 7 days postmarathon. Low-intensity exercise during the recovery week did not significantly differentiate the exercise group from the rest group.

This article has been cited by other articles:

				H. W. Goforth Jr., D. Laurent, W. K. Prusaczyk, K. E. Schneider, K. F. Petersen, and G. I. Shulman Effects of depletion exercise and light training on muscle glycogen supercompensation in men Am J Physiol Endocrinol Metab, December 1, 2003; 285(6): E1304 - E1311. [Abstract] [Full Text] [PDF]

				L. J. van Loon, W. H. Saris, M. Kruijshoop, and A. J. Wagenmakers Maximizing postexercise muscle glycogen synthesis: carbohydrate supplementation and the application of amino acid or protein hydrolysate mixtures Am. J. Clinical Nutrition, July 1, 2000; 72(1): 106 - 111. [Abstract] [Full Text] [PDF]

				B. F. Hansen, W. Derave, P. Jensen, and E. A. Richter No limiting role for glycogenin in determining maximal attainable glycogen levels in rat skeletal muscle Am J Physiol Endocrinol Metab, March 1, 2000; 278(3): E398 - E404. [Abstract] [Full Text] [PDF]

				S. Asp, J. R. Daugaard, T. Rohde, K. Adamo, and T. Graham Muscle glycogen accumulation after a marathon: roles of fiber type and pro- and macroglycogen J Appl Physiol, February 1, 1999; 86(2): 474 - 478. [Abstract] [Full Text] [PDF]

				S. Asp, T. Rohde, and E. A. Richter Impaired muscle glycogen resynthesis after a marathon is not caused by decreased muscle GLUT-4 content J Appl Physiol, November 1, 1997; 83(5): 1482 - 1485. [Abstract] [Full Text] [PDF]

				H. W. Goforth, Jr., D. A. Arnall, B. L. Bennett, and P. G. Law Persistence of supercompensated muscle glycogen in trained subjects after carbohydrate loading J Appl Physiol, January 1, 1997; 82(1): 342 - 347. [Abstract] [Full Text] [PDF]

				W. M. Sherman and D. L. Costill The marathon: Dietary manipulation to optimize performance Am. J. Sports Med., January 1, 1984; 12(1): 44 - 51. [Abstract] [PDF]