Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 54: 1287-1291, 1983;
8750-7587/83 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zijlstra, W. G.
Right arrow Articles by Zwart, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zijlstra, W. G.
Right arrow Articles by Zwart, A.

Journal of Applied Physiology, Vol 54, Issue 5 1287-1291, Copyright © 1983 by American Physiological Society

ARTICLES

Molar absorptivities of human hemoglobin in the visible spectral range

W. G. Zijlstra, A. Buursma and A. Zwart

In a recent paper, Burkhard and Barnikol (J. Appl. Physiol.: Respirat. Environ. Exercise Physiol. 52: 124-130, 1982) claimed that the absorption spectra of human (oxy-)hemoglobin are dependent on the total hemoglobin concentration (CHb) and it is suggested that this might also be the case with cyanmethemoglobin (HiCN). Such relationships would invalidate the widely used spectrophotometric methods for the determination of total hemoglobin and the fractions of various hemoglobin derivatives in human blood. Although Burkhard and Barnikol's findings are rather improbable considering earlier data, we measured the millimolar absorptivities of oxyhemoglobin (epsilon HbO2) and cyanmethemoglobin (epsilon HiCN) at various wavelengths over a wide range of concentrations (CHb approximately equal to 0.004-10 mmol x 1(-1)), using two different types of spectrophotometers. epsilon HbO2 and epsilon HiCN proved to be independent of CHb. Moreover the values obtained confirmed those in the earlier literature, whereas those of Burkhard and Barnikol are some 30% higher. Consequently there is no reason to doubt the validity of the generally accepted millimolar absorptivities of human hemoglobin.


This article has been cited by other articles:


Home page
 J. Exp. Biol.Home page
J. B. Andersen, M. S. Hedrick, and T. Wang
Cardiovascular responses to hypoxia and anaemia in the toad Bufo marinus
J. Exp. Biol., March 1, 2003; 206(5): 857 - 865.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Busk, F. B. Jensen, and T. Wang
Effects of feeding on metabolism, gas transport, and acid-base balance in the bullfrog Rana catesbeiana
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2000; 278(1): R185 - R195.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. W. Hicks and T. Wang
Hypoxic hypometabolism in the anesthetized turtle, Trachemys scripta
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 1999; 277(1): R18 - R23.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online