Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 53: 1138-1143, 1982;
8750-7587/82 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Klocke, R. A.
Right arrow Articles by Flasterstein, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Klocke, R. A.
Right arrow Articles by Flasterstein, F.

Journal of Applied Physiology, Vol 53, Issue 5 1138-1143, Copyright © 1982 by American Physiological Society

ARTICLES

Kinetics of erythrocyte penetration by aliphatic acids

R. A. Klocke and F. Flasterstein

The rate of entry of aliphatic acids into human red blood cells was studied using a new stopped-flow technique. Solutions of aliphatic acids and erythrocyte suspensions were rapidly mixed in a stopped-flow reaction apparatus. The change in extracellular pH resulting from entry of acid into the cell was monitored using chlorophenol red as an extracellular indicator. Extracellular pH of the mixture was linearly related to differential light transmission at 480 and 560 nm. Values of membrane permeability were fitted to data using a model of passive exchange with flux proportional to the transmembrane concentration gradient. At all temperatures the permeabilities were in the order: valeric acid greater than butyric acid greater than propionic acid. Permeabilities were highly temperature dependent and the activation energy (10.5 kcal/mol) remained constant between 7 and 37 degrees C. Despite rapid rates of exchange, permeability measurements did not exhibit evidence of artifact secondary to an unstirred solvent layer on the cell surface. The data are compatible with the hypothesis that aliphatic acid transport across the erythrocyte membrane is passive in nature and proportional to the transmembrane concentration gradient.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online