Journal of Applied Physiology Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 53: 87-95, 1982;
8750-7587/82 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reeves, R. B.
Right arrow Articles by Olszowka, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reeves, R. B.
Right arrow Articles by Olszowka, A. J.

Journal of Applied Physiology, Vol 53, Issue 1 87-95, Copyright © 1982 by American Physiological Society

ARTICLES

Oxygen affinity and Bohr coefficients of dog blood

R. B. Reeves, J. S. Park, G. N. Lapennas and A. J. Olszowka

Complete dynamic oxygen equilibrium curves (O2EC) on dog whole blood were measured at 25 and 39 degrees C using a spectrophotometric micro blood film technique. O2EC were run at three CO2 levels (2, 4, and 8%) for each of three base excess levels (-10, 0, +10 meq/l). The standard curve (ph 7.4) was determined for saturations 0-0.98. At 39 degrees C the standard curve O2 pressure at half-saturation (P50) was 31.5 Torr; fixed-acid Bohr factor, -0.488; CO2 Bohr factor, -0.498; delta log P50/delta log PCO2, -0.0045. CO2 Bohr slope was linear over the pH range of 7-8. Bohr factors were not significantly saturation dependent. At 25 degrees C P50 was 15.4 Torr and CO2 Bohr factor, -0.647. The temperature coefficient (delta log P50/delta T) equaled 0.022. Dog O2EC were shown with curve-fitting techniques to be isomorphic with human blood O2EC. The absence of significant oxylabile carbamate formation in dog red blood cells (RBC) was attributed to high 2,3-diphosphoglycerate (DPG) concentrations, 6.23 mM/l RBC, equal to a DPG/Hb4 ratio of 1.12. A simple two-constant equation S = [(37,900)/(P3 + 205P) + 1]-1, where S is saturation and P is oxygen tension, was found to fit the dog 39 degrees C standard curve.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G. Gong and J. Zhang
Transmural metabolic heterogeneity at high cardiac work states
Am J Physiol Heart Circ Physiol, July 1, 1999; 277(1): H236 - H242.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
Y. Ishibashi, D. J. Duncker, J. Zhang, and R. J. Bache
ATP-Sensitive K+ Channels, Adenosine, and Nitric Oxide–Mediated Mechanisms Account for Coronary Vasodilation During Exercise
Circ. Res., February 23, 1998; 82(3): 346 - 359.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. E. Curtis, T. A. Walker, W. E. Bradley, and S. M. Cain
Raising P50 increases tissue PO2 in canine skeletal muscle but does not affect critical O2 extraction ratio
J Appl Physiol, November 1, 1997; 83(5): 1681 - 1689.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
J. Zhang, C. Toher, M. Erhard, Y. Zhang, K. Ugurbil, R. J. Bache, T. Lange, and D. C. Homans
Relationships Between Myocardial Bioenergetic and Left Ventricular Function in Hearts With Volume-Overload Hypertrophy
Circulation, July 1, 1997; 96(1): 334 - 343.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online