Journal of Applied Physiology, Vol 51, Issue 5 1268-1277, Copyright © 1981 by American Physiological Society

ARTICLES

Total lung lymph flow and fluid compartmentation in edematous dog lungs

J. C. Parker, M. Crain, F. Grimbert, G. Rutili and A. E. Taylor

The effect of fluid volume loading on lung tissue fluid compartments and pulmonary lymph flow was studied in 7 dogs. A bolus of 125I-labeled albumin was administered 1 h after a 10--15% body weight Tyrode infusion. Then concentrations of labeled and endogenous albumin in pulmonary lymph and plasma were monitored for 4--6 h. The time course of plasma and lymph [125I]albumin specific activities was analyzed using kinetic and both the linear and nonlinear solute flux equations. Plasma specific activity exhibited a two-component decay with mean rate constants of 2.65 and 0.071 h-1. Albumin equilibrated between plasma and lymph at a rate of 0.327 h-1, or with a half time of 2.12 h. For albumin, the mean permeability-surface area product was 0.043 ml/min, and total distribution volume was 22.6 ml. This indicated that the cannulated lymphatics drained 25% of total lung weight, and that lung lymph flow was 0.063 ml . min-1 . 100 g-1 in normally hydrated lungs, and 0.225 ml . min-1 . 100 g-1 in edematous lungs. During edema the extravascular 99mTc-DTPA (diethylenetriamine pentaacetic acid) space increased by 79% and the total extravascular lung water by 40%. The extravascular albumin space was only one-third that predicted for the extent of edema. This indicates a significant volume of edema fluid sequestered in tissue compartments, such as perivascular cuffs and alveolar spaces, which did not equilibrate rapidly with capillary filtrate draining into the pulmonary lymphatics.

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