Journal of Applied Physiology, Vol 47, Issue 4 706-711, Copyright © 1979 by American Physiological Society
Selected lobar injury after infusion of oleic acid
B. P. Griffith, R. G. Carroll, R. L. Hardesty, R. L. Peel and H. S. Borovetz
A model of respiratory distress has been developed using sheep in which an
isolated lobar injury is induced by infusion of oleic acid into a selected
lobar artery. The directed insult permits acute and chronic phase study
without the requirement of intensive pulmonary support. One hour after
selective lobar injury the affected pulmonary venous oxygenation (Ppvo2)
was reduced from greater than 310 to 66-90 Torr. Ppvo2 was most impaired at
48 h (40-55 Torr), but showed improvement by 96 h postinjury. Radionuclide
estimates of lobar ventilation and perfusion demonstrated an initial fall
in ventilation by 51% and corresponding 41% reduction in perfusion. By 24 h
the depression of perfusion matched that of ventilation. Sequential light
and scanning electron microscopy demonstrated the presence of a
nonhomogeneous injury with areas of both complete parenchymal replacement
by fibrous tissue and those with minimal architectural distortion. The
selective injury model offers a useful methodology for the evaluation of
the effects of various agents on the acute and chronic response of the lung
to injury.
