Journal of Applied Physiology, Vol 46, Issue 3 447-450, Copyright © 1979 by American Physiological Society
Reversal of 2-deoxyglucose inhibition of serotonin uptake in isolated guinea pig lung
A. B. Fisher, H. Steinberg and C. Dodia
The effect of various substrates on the rate of uptake of
5-hydroxytryptamine (serotonin) from the pulmonary circulation was studied
with isolated guinea pig lungs. Lungs were ventilated and were perfused
with an electrolyte solution in a recirculating system. Uptake of serotonin
was calculated from the rate of disappearance of [14C]serotonin from the
perfusate. Serotonin uptake was inhibited by approximately 40% when 5 mM
2-deoxyglucose was substituted for glucose in the pulmonary perfusate. The
inhibition due to 2-deoxyglucose was reversed by addition to the perfusate
of pyruvate, lactate, acetate, alanine, beta-hydroxybutyrate, palmitate, or
glycerol. Fructose ans succinate were less efficient substrates while no
significant effect was noted with L-glycerol 1-phosphate or
L-phenylalanine. These results indicate that a wide variety of
metabolizable substrates can be utilized by the pulmonary endothelium to
support uptake of serotonin.
