Journal of Applied Physiology
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J Appl Physiol 44: 818-820, 1978;
8750-7587/78 $5.00
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Journal of Applied Physiology, Vol 44, Issue 5 818-820, Copyright © 1978 by American Physiological Society


ARTICLES

Measurement of blood O2 and CO2 concentrations using PO2 and PCO2 electrodes

A. L. Harabin and L. E. Farhi

Accurate dilution of a small blood volume with a carbon monoxide-saturated solution allows measurement of the whole blood O2 concentration as an increase in O2 tension in the solution. We have improved the method by simplifying both equipment and procedure. We also suggest an additional step in which the mixture is acidified, thereby allowing the measurement of CO2 concentration in the same solution with a CO2 electrode. The accuracy of both the O2 and CO2 determinations compares favorably with that obtained with other micromethods.





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