Journal of Applied Physiology, Vol 43, Issue 4 714-720, Copyright © 1977 by American Physiological Society
End-respiratory pressure ventilation and sulfobromophthalein sodium excretion in dogs
E. E. Johnson, J. Hedley-Whyte and S. V. Hall
Sulfobromophthalein sodium (BSP) 25 mg/kg body wt was given as a single iv
injection to 32 fasted dogs. Serum samples at 3, 5, 10, 20, 30, 45, 60, 80,
and 120 min postinjection were analyzed for total concentration of BSP and
from 30 to 120 min for percent conjugated BSP. Four groups were compared:
spontaneous ventilation; intermittent positive-pressure ventilation (IPPV)
and continuous positive-pressure ventilation (CPPV) (2 groups). During
CPPV, one group of dogs was given a continuous infusion of cholecystokinin
octapeptide (CCK-8, 1 ng/kg per min). Central venous pressure averaged 11.3
+/- 0.7 (SE) cmH2O in dogs with CPPV + CCK-8 and 11.8 +/- 0.8 (SE) cmH2O in
dogs with CPPV alone. At 3, 5, and 10 min postinjection serum BSP levels
were similar in all groups. From 30 to 120 min postinjection serum levels
of both free and conjugated BSP were higher in dogs ventilated with CPPV
alone than in any other group (P less than 0.01). Dogs given CCK-8 during
CPPV had serum BSP levels that were statistically similar to dogs breathing
spontaneously or ventilated with IPPV. We conclude that CPPV impairs BSP
excretion. This effect is counteracted by CCK-8.
