Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 41: 480-488, 1976;
8750-7587/76 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Harbig, K.
Right arrow Articles by Reivich, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Harbig, K.
Right arrow Articles by Reivich, M.

Journal of Applied Physiology, Vol 41, Issue 4 480-488, Copyright © 1976 by American Physiological Society

ARTICLES

In vivo measurement of pyridine nucleotide fluorescence from cat brain cortex

K. Harbig, B. Chance, A. G. Kovach and M. Reivich

A modification of the methods is described which makes it possible to measure pyridine nucleotide fluorescence from the brain cortex in vivo without interference from movement and hemodynamic artifacts. Movement artifacts were eliminated by the use of a window technique. Fluorescence changes due to changes in hemoglobin oxygenation have been eliminated by measuring fluorescence at an isobestic wavelength of the hemoglobin-oxyhemoglobin reaction. The interference due to changes in red blood cell concentration has been studied by simultaneous measurements of fluorescence and ultraviolet reflection. Hemodilution revealed a linear relationship between the fluorescence from the pyridine nucleotide and reflected ultraviolet light. The ratio between the light absorption changes was approximately unity under the particular optical geometry employed in this study. This method has been used to measure fluorescence changes produced by nitrogen anoxia. The technique is discussed in relation to previous methods and the effects of anoxia are compared to previous findings.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Mayevsky and G. G. Rogatsky
Mitochondrial function in vivo evaluated by NADH fluorescence: from animal models to human studies
Am J Physiol Cell Physiol, February 1, 2007; 292(2): C615 - C640.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
A. Takahashi, P. Camacho, J. D. Lechleiter, and B. Herman
Measurement of Intracellular Calcium
Physiol Rev, October 1, 1999; 79(4): 1089 - 1125.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. Toth, T. Ivanics, Z. Ruttner, D. W. Slaaf, R. S. Reneman, and L. Ligeti
Quantitative assessment of [Ca2+]i levels in rat skeletal muscle in vivo
Am J Physiol Heart Circ Physiol, November 1, 1998; 275(5): H1652 - H1662.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
R. Kramer and R. Pearlstein
Cerebral cortical microfluorometry at isosbestic wavelengths for correction of vascular artifact
Science, August 17, 1979; 205(4407): 693 - 696.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online