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J Appl Physiol 40: 260-262, 1976;
8750-7587/76 $5.00
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Journal of Applied Physiology, Vol 40, Issue 2 260-262, Copyright © 1976 by American Physiological Society

ARTICLES

Construction of picoliter-nanoliter self-filling volumetric pipettes

P. M. Quinton

A technique is described whereby-volume, self-filling micropipettes can be constructed with minimal time and effort. The delivery volumes of such pipettes may range from several picoliters to more than 100 nl. The delivery of a given pipette of even the smallest volume is precise, generally yielding standard deviations much less than 1% of the mean volume as measured by liquid-scintillation counting of isotope dilutions. The technique offers considerable advantage to previously described techniques for constructing small constant-volume pipettes.


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