Journal of Applied Physiology
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J Appl Physiol 39: 860-862, 1975;
8750-7587/75 $5.00
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Journal of Applied Physiology, Vol 39, Issue 5 860-862, Copyright © 1975 by American Physiological Society

ARTICLES

A technique for measurement of retrograde coronary blood flow in intact anesthetized dogs

K. Afonso, G. L. Friede, F. M. Blau, G. T. Bandow and G. G. Rowe

A technique for measurement of retrograde coronary blood flow in intact anesthetized dogs is described. Occlusion of the coronary artery is produced by the inflation of a small rubber balloon at the tip of a no. 9 cardiac catheter placed under fluorescopy in a branch of the left coronary artery. Blood which bleeds back from the occluded coronary artery through the no. 9 catheter is diverted into a small reservoir of 1-ml capacity. The time to fill this reservoir is recorded electrically. Retrograde coronary blood flow is calculated from the time required to fill this reservoir. Results indicate good repeatability of meadurements. The technique seems to be a simple, adequate, and convenient means for assessing agents for possible vasodilator action on the collateral circulation in intact animals.




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