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J Appl Physiol 39: 145-149, 1975;
8750-7587/75 $5.00
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Journal of Applied Physiology, Vol 39, Issue 1 145-149, Copyright © 1975 by American Physiological Society

ARTICLES

A simple fluorescent method for quantitative determination of aortic protein uptake

M. E. Katora and T. M. Hollis

A quantitative system for direct protein tracing and measurement of net protein uptake in the aorta using fluorescein isothiocyanate conjugated bovine serum albumin (FITCBSA) is described. Using Wistar rats, a mean aortic FITCBSA net uptake of 29.7 times 10(-17) g FITCBSA per mum2 aortic endothelial surface area per 24 h was obtained. Intra-aortic localization of the FITCBSA was observed along the endothelium and the collagen-elastin bands. The values obtained using this FITCBSA system are comparable with those of a previously established isotopic technique measuring aortic albumin flux and reconfirm the previous findings of the existence of an albumin permeability gradient in the thoracic aorta.




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