Journal of Applied Physiology Information on EB 2010
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J Appl Physiol 38: 1140-1142, 1975;
8750-7587/75 $5.00
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Journal of Applied Physiology, Vol 38, Issue 6 1140-1142, Copyright © 1975 by American Physiological Society

ARTICLES

A simplified method for determining the P50 of blood

B. W. Kirk, M. B. Raber and K. R. Duke

A method to determine the P50 of whole blood is described using a modified American Optical reflectance oximeter, pump, and membrane tonometer, together with PO2, PCO2, and pH measurements in a standard blood gas machine. Determinations of P50 were made in 66 patients and normal subjects and in two situations where P50 was very low and very high. The results were compared to oxygen saturations calculated from measured oxygen content. The directly determined oxygen saturation agreed with the assumed saturation of 50 per cent in the oximeter within 0.5 per cent. The apparatus appears to be a simple and relatively inexpensive method to obtain P50 as long as blood carboxyhemoglobin or methemoglobin contents are not elevated.




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