Journal of Applied Physiology
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J Appl Physiol 20: 637-646, 1965;
8750-7587/65 $5.00
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Role of diffusion of oxygen in the respiration of tissues at different temperatures

Douglas A. Farr 1 and Frederick A. Fuhrman 1

1 Max C. Fleischmann Laboratories of the Medical Sciences, School of Medicine, Stanford University, Palo Alto, California

The hypothesis is presented that rates of respiration of sheets and slices of mammalian tissues as measured in vitro are usually lower than the true ones because of inadequate oxygenation in thick preparations and excessive damage to cells in thin ones. Data to support the hypothesis were obtained by a study of Qo2 as a function of temperature. When particles were small enough (avian red blood cells and brain homogenates) that diffusion of oxygen did not limit Qo2, a plot of log Qo2 versus 1/T (T = absolute temperature) yielded a straight line. However, when sheets and slices of tissues were used, such plots yielded lines whose slopes decreased with increase in temperature. For rat diaphragm, oxygenation appeared to be inadequate above 18 C. For rat liver, equations are presented for correcting Qo2 for damage caused by slicing and for inadequate oxygenation at higher temperatures. Data for respiration of tissues in 10% oxygen and at 2 atm oxygen pressure supported the hypothesis.

oxygen tension; tissue slices—limiting thickness; tissue slices—thickness and Qo2

Submitted on May 5, 1964




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