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J Appl Physiol 20: 571-572, 1965;
8750-7587/65 $5.00
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A system for providing surgical anesthesia for germfree rodents

Darryl Carter 1 and Albert Einheber 1

1 Department of Germfree Research, Division of Basic Surgical Research, Walter Reed Army Institute of Research, Washington, D. C.

A system which has been successfully used to provide anesthesia for experimental surgical procedures on germfree rodents within a germfree surgical isolator is described. The preselected air-halothane mixture is sterilized by passage through spun-glass filters. A small Lucite chamber and a nose cone allow induction and maintenance of the halothane anesthesia within the isolator. The system has provided a safe, reproducible anesthesia from which recovery is brief and from which there is no explosion hazard.

germfree animal techniques; halothane anesthesia

Submitted on October 15, 1964






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