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J Appl Physiol 19: 315-318, 1964;
8750-7587/64 $5.00
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Thin-layer chromatography of component A of the menstrual stimulant

H. J. Clitheroe 1

1 Department of Physiology, University of Sheffield, Sheffield, England, and Bureau of Biological Research, Rutgers—The State University, New Brunswick, New Jersey

Thin-layer chromatography has been adapted to separate material of high biological potency from much of the inactive material contained in component A of the menstrual stimulant. The chromatoplates were developed with solvent mixture of chloroform, methanol, and water (60:10:1). Visualization of the spots was carried out by various methods; ultraviolet fluorescence of the charred material appeared to be the most sensitive. Samples of ricinoleic and phloionolic acids were used as reference standards. The biological active material of the component A preparations always ran just in front of phloionolic acid. Menstrual fluid was collected in vaginal cups; total volume and crude liquid extract measurements were also carried out on the fluid.

uterine smooth muscle stimulant; menstrual flow rates

Submitted on October 1, 1963






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