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J Appl Physiol 18: 1041-1042, 1963;
8750-7587/63 $5.00
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Continuous recording of arteriolar dimensions with a television microscope

Curt A. Wiederhielm 1

1 Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington

A system which permits continuous recording of dimensions of microscopic blood vessels is described. The system utilizes information contained in the video signal of a television microscope to develop an analog voltage proportional to the time required for the electron beam to sweep across the image of the blood vessel. This time interval is also proportional to the dimension of the vessel. Calibration of the system yielded a standard error of estimate of ±3.7 µ on a series of glass capillaries, ranging in size from 15 to 150 µ. The rise time of the system was in the order of 40 msec. Long- and short-term drift was less than 3 µ/hr. The system is used in an experimental study of viscoelastic properties of small arteries and arterioles.

microcirculation; viscoelastic properties; frog mesentery

Submitted on April 17, 1963




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