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J Appl Physiol 18: 325-328, 1963;
8750-7587/63 $5.00
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Anticoagulant effects of concentrated cephalin in the thromboplastin generation test

Kenneth J. Coffield 1 and Mary Ann Cocanour 1

1 Institute of Medical Research of The Toledo Hospital, Toledo, Ohio

Concentrated suspensions of phospholipid (cephalin) inhibit thromboplastin generation as a result of adsorption or inactivation of factors V and VIII by the phospholipid. Such inhibition can be overcome by increasing the concentration of plasma and serum reagents, or allowing product I to form prior to the addition of phospholipid. Adsorption of plasma reagents with washed sedimented cephalin demonstrates directly the depletion of factors V and VIII.

Submitted on July 5, 1962






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