Journal of Applied Physiology http://www.adinstruments.com/labchart/faseb
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 15: 520-524, 1960;
8750-7587/60 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bender, M. A.
Right arrow Articles by Smith, L. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bender, M. A.
Right arrow Articles by Smith, L. H.

Preservation of viable bone marrow cells by freezing

M. A. Bender 1, Phan The Tran 1, and L. H. Smith 1

1 Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee

In a study of low-temperature preservation, mouse bone marrow cells were slow-frozen and fast-thawed. A number of additives, including glycerol and other polyhydric alcohols, were tested for their ability to protect the cells against freezing and thawing damage. Effectiveness of thawed cells to promote recovery in lethally irradiated (900 r) mice was used as a measure of viability. Tyrode's solution, mouse serum, glucose, polyvinylpyrrolidone, dextran and bovine serum albumin gave little or no protection at the concentrations tested. Polyhydric alcohols and some sugars and other organic compounds were effective at certain concentrations. Results of tests of marrow frozen in 15% glycerol made after storage at various temperatures for various times indicate that liquid nitrogen temperatures give the best long-term storage. The eosin uptake test was found to be a poor assay of the effectiveness of thawed marrow samples.

Submitted on November 13, 1959




This article has been cited by other articles:


Home page
 Arch Intern MedHome page
J. W. FERREBEE and E. D. THOMAS
Transplantation of Marrow in Man
Arch Intern Med, October 1, 1960; 106(4): 523 - 531.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online