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J Appl Physiol 14: 27-30, 1959;
8750-7587/59 $5.00
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Relationship between nonprotein sulfhydryl concentration of seminal fluid and motility of spermatozoa in man

Fritz M. Haag 1 and John MacLeod 1

1 Sterility Research Station, Lexington, Kentucky, and Department of Anatomy, Cornell University Medical College, New York City

The nonprotein sulfhydryl content of human seminal fluid was measured and an inverse correlation between the SH titer and the motility of the spermatozoa in the fluid over a 5-hour period was found. The same inverse correlation, with less significance, was shown between the SH titer and the percentage of active cells, semen quality and volume of ejaculates. No correlation between the SH concentration and the sperm count was detectable. The nonprotein SH titer was identified qualitatively and quantitatively as ergothioneine. The concentration in human seminal fluid is 1.5 mg %, a value close to that found in human blood.

Submitted on July 17, 1958







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