Journal of Applied Physiology
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J Appl Physiol 14: 133-136, 1959;
8750-7587/59 $5.00
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Use of Triton X-100 as a hemolytic agent in the spectrophotometric measurement of blood O2 saturation

Gladys E. Deibler 1, Mary S. Holmes 1, Phyllis L. Campbell 1, and June Gans 1

1 Section on Cerebral Metabolism, Laboratory of Clinical Science, National Institute of Mental Health, Bethesda, Maryland

A spectrophotometric method for the measurement of blood O2 saturation utilizing Triton X-100 as the hemolytic agent is described. The results of its comparison with the standard manometric method indicate that it provides reliable values for blood O2 saturation. Triton X-100 was found to be a more suitable hemolytic agent than saponin. A nomogram is presented which facilitates the calculation of percentage of O2 saturation from the measurements required by the method.

Submitted on August 27, 1958




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