Journal of Applied Physiology
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J Appl Physiol 12: 65-70, 1958;
8750-7587/58 $5.00
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Comparative Measurements With a New Procedure for Measuring the Blood Oxygen Tension in Vitro

Ferdinand Kreuzer 1, T. Richard Watson JR. 1, and James M. Ball 1

1 From the Department of Physiology, Dartmouth Medical School, the Cardio-Pulmonary Laboratory of the Hitchcock Foundation, and the Mary Hitchcock Memorial Hospital, Hanover, New Hampshire

A new method for measuring the oxygen tension of the blood, using Clark's platinum electrode and applying direct recording, is described. The data obtained with this procedure are compared with a) adjusted tonometer equilibrations with determination of the oxygen in the gas phase by the Van Slyke technique and b) data obtained from determinations by the Riley technique. The standard deviation of the polarographic method is 3.0 mm Hg as compared with 3.28 mm Hg for the Riley technique and 1.89 mm Hg for the Van Slyke gas determinations in our hands. The regression line of the polarographic values against the tonometer gas data has a slope of 0.997, and that against the Riley determinations (uncorrected) is very close to 1.0.

Note:
with the technical assistance of Dorothy M. Donnelly, D. S. Imredy, T. C. Jackson and D. C. Dixon

Submitted on August 19, 1957




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F. KREUZER and C. G. NESSLER JR.
Method of Polarographic in vivo Continuous Recording of Blood Oxygen Tension
Science, October 24, 1958; 128(3330): 1005 - 1006.
[Abstract] [PDF]


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