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J Appl Physiol 11: 365-370, 1957;
8750-7587/57 $5.00
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An Improved Polarographic Method for Measuring Oxygen Tension in Whole Blood

Brian J. Sproule 1, William F. Miller 1, Ivan E. Cushing 1, and Carleton B. Chapman 1

1 From the Cardiopulmonary Laboratory, Department of Internal Medicine, University of Texas Southwestern Medical School, and Parkland Memorial Hospital, Dallas, Texas

A new type of polarographic electrode (Clark) was found to provide a means of accurately and rapidly determining the O2 tension of whole blood at all levels of Po2. The electrode response is independent of ph, CO2 tension and hematocrit. Temperature and pressure changes produce predictable linear variations in electrode response for any given oxygen tension. There were found to be occasional marked variations in calibration curves for unknown reasons. Thus, accuracy can only be assured by developing a calibration curve for each subject's blood. A simple system for calibration of the electrode in whole blood has been described.

Submitted on June 14, 1957




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F. KREUZER and C. G. NESSLER JR.
Method of Polarographic in vivo Continuous Recording of Blood Oxygen Tension
Science, October 24, 1958; 128(3330): 1005 - 1006.
[Abstract] [PDF]


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