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J Appl Physiol 11: 136-138, 1957;
8750-7587/57 $5.00
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Intestinal Absorption by Perfusion in Situ

F. A. Jacobs 1 and M. Luper 1

1 From the Guy and Bertha Ireland Research Laboratory, Department of Biochemistry, University of North Dakota Medical School, Grand Forks, North Dakota

An apparatus and technique for the perfusion of a specific cannulated segment of small intestine of the rat, in situ, have been described. By the removal and analysis of perfusate samples from the system, continuous intestinal absorption curves have been established from a single animal in the steady state. These data agree with load determinations. Data based upon the intestinal uptake of glucose and xylose show that this system lends itself to in situ studies on absorption.

Submitted on November 15, 1956






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