HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 107/4/1308    most recent 00348.2009v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Google Scholar Articles by Smith, G. I. Articles by Mittendorfer, B. PubMed PubMed Citation Articles by Smith, G. I. Articles by Mittendorfer, B.

TRANSLATIONAL PHYSIOLOGY

No major sex differences in muscle protein synthesis rates in the postabsorptive state and during hyperinsulinemia-hyperaminoacidemia in middle-aged adults

¹Washington University, School of Medicine, St. Louis, Missouri; and ; ²University of Nottingham, School of Graduate Entry Medicine and Health, Derby, United Kingdom

Submitted 2 April 2009 ; accepted in final form 29 July 2009

Men have more muscle than women, but most studies evaluating sex differences in muscle protein metabolism have been unable to discern sexual dimorphism in basal muscle protein turnover rates in young and middle-aged adults. We hypothesized that the anabolic response to nutritional stimuli (i.e., amino acids and insulin) would be greater in young/middle-aged men than women. We therefore measured the rates of muscle protein synthesis (MPS) in 16 healthy individuals [8 men and 8 women, matched for age (mean ± SE: 37.7 ± 1.5 yr) and body mass index (25.2 ± 0.7 kg/m²)] after an overnight fast (plasma insulin 5 µU/ml and plasma phenylalanine 60 µM) and during a hyperinsulinemic-hyperaminoacidemic-euglycemic clamp (plasma insulin 28 µU/ml; plasma phenylalanine 110 µM; plasma glucose 5.4 mM). The rates of MPS were not different between men and women (ANOVA main effect for sex; P = 0.49). During the clamp, the rate of MPS increased by 50% (P = 0.003) with no difference in the increases from basal values between men and women (+0.019 ± 0.004 vs. +0.018 ± 0.010%/h, respectively; P = 0.93). There were also no differences between men and women in the basal concentrations of muscle phosphorylated Akt^Ser473, Akt^Thr308, mTOR^Ser2448, and p70s6k^Thr389 or in the hyperinsulinemia-hyperaminoacidemia-induced increases in phosphorylation of those signaling elements (P 0.25). We conclude that there are no major differences in the rate of MPS and its intracellular control during basal conditions and during hyperinsulinemia-hyperaminoacidema between young and middle-aged adult men and women.

signal transduction; protein metabolism

This article has been cited by other articles:

				G. I. Smith, D. T. Villareal, C. P. Lambert, D. N. Reeds, B. S. Mohammed, and B. Mittendorfer Timing of the initial muscle biopsy does not affect the measured muscle protein fractional synthesis rate during basal, postabsorptive conditions J Appl Physiol, February 1, 2010; 108(2): 363 - 368. [Abstract] [Full Text] [PDF]