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J Appl Physiol 105: 1830-1836, 2008. First published October 16, 2008; doi:10.1152/japplphysiol.90955.2008
8750-7587/08 $8.00
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Contribution of IL-6 to the Hsp72, Hsp25, and {alpha}β-crystallin responses to inflammation and exercise training in mouse skeletal and cardiac muscle

Kimberly A. Huey and Benjamin M. Meador

Department of Kinesiology, University of Illinois at Urbana-Champaign, Urbana, Illinois

Submitted 24 July 2008 ; accepted in final form 12 October 2008

The heat shock proteins (Hsps) Hsp72, Hsp25, and {alpha}β-crystallin ({alpha}βC) may protect tissues during exercise and/or inflammatory insults; however, no studies have investigated whether exercise training increases both basal and inflammation-induced expression of these Hsps in skeletal or cardiac muscle. IL-6 is produced by muscle during both exercise and inflammation and has been shown to modulate Hsp expression. These studies tested the hypothesis that voluntary wheel running (RW) increases basal and inflammation-induced Hsp72, Hsp25, and {alpha}βC protein through an IL-6-dependent mechanism. We compared Hsp72, Hsp25, {alpha}βC, and IL-6 protein levels 4 h after systemic inflammation induced by lipopolysaccharide (LPS) in skeletal and cardiac muscles of wild-type (IL-6+/+) and IL-6 deficient (IL-6–/–) mice after 2 wk of RW or normal cage activity (Sed). LPS significantly increased skeletal Hsp72 and Hsp25 relative to saline in Sed IL-6+/+, but not IL-6–/– mice. LPS increased Hsp72 relative to saline in Sed IL-6+/+ cardiac muscle. RW increased basal Hsp72, Hsp25, and {alpha}βC in skeletal muscle in IL-6+/+ and IL-6–/– mice. However, LPS was not associated with increases in any Hsp in RW IL-6+/+ or IL-6–/– mice. LPS increased IL-6 protein in skeletal muscle and plasma in Sed and RW groups, with a significantly greater response in RW. The major results provide the first in vivo evidence that the absence of IL-6 is associated with reduced skeletal muscle Hsp72 and Hsp25 responses to LPS, but that IL-6 is not required for exercise-induced Hsp upregulation in skeletal or cardiac muscle.

heat shock proteins; interleukin-6; lipopolysaccharide


Address for reprint requests and other correspondence: K. A. Huey, 120 Freer Hall, 906 S. Goodwin Ave., Urbana, IL 61801 (e-mail: khuey{at}illinois.edu)






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