HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 105/4/1098    most recent 00847.2007v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Mathai, A. S. Articles by Graham, T. E. Search for Related Content PubMed PubMed Citation Articles by Mathai, A. S. Articles by Graham, T. E.

Rapid exercise-induced changes in PGC-1 mRNA and protein in human skeletal muscle

Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada

Submitted 7 August 2007 ; accepted in final form 15 July 2008

The mRNA of the nuclear coactivator peroxisome proliferator-activated receptor- coactivator-1 (PGC-1) increases during prolonged exercise and is influenced by carbohydrate availability. It is unknown if the increases in mRNA reflect the PGC-1 protein or if glycogen stores are an important regulator. Seven male subjects [23 ± 1.3 yr old, maximum oxygen uptake (O_{2 max}) 48.4 ± 0.8 ml·kg^–1·min^–1] exercised to exhaustion (2 h) at 65% O_{2 max} followed by ingestion of either a high-carbohydrate (HC) or low-carbohydrate (LC) diet (7 or 2.9 g·kg^–1·day^–1, respectively) for 52 h of recovery. Glycogen remained depressed in LC (P < 0.05) while returning to resting levels by 24 h in HC. PGC-1 mRNA increased both at exhaustion (3-fold) and 2 h later (6.2-fold) (P < 0.05) but returned to rest levels by 24 h. PGC-1 protein increased (P < 0.05) 23% at exhaustion and remained elevated for at least 24 h (P < 0.05). While there was no direct treatment effect (HC vs. LC) for PGC-1 mRNA or protein, there was a linear relationship between the changes in glycogen and those in PGC-1 protein during exercise and recovery (r = –0.68, P < 0.05). In contrast, PGC-1β did not increase with exercise but rather decreased (P < 0.05) below rest level at 24 and 52 h, and the decrease was greater (P < 0.05) in LC. PGC-1 protein content increased in prolonged exercise and remained upregulated for 24 h, but this could not have been predicted by the changes in mRNA. The β-isoform declined rather than increasing, and this was greater when glycogen was not resynthesized to rest levels.

carbohydrate; gene expression; glucose; insulin; training signals; metabolism

This article has been cited by other articles:

				T. Wenz, F. Diaz, D. Hernandez, and C. T. Moraes Endurance exercise is protective for mice with mitochondrial myopathy J Appl Physiol, May 1, 2009; 106(5): 1712 - 1719. [Abstract] [Full Text] [PDF]

				L. N. Sutherland, M. R. Bomhof, L. C. Capozzi, S. A.U. Basaraba, and D. C. Wright Exercise and adrenaline increase PGC-1{alpha} mRNA expression in rat adipose tissue J. Physiol., April 1, 2009; 587(7): 1607 - 1617. [Abstract] [Full Text] [PDF]

				M. Hoene, R. Lehmann, A. M. Hennige, A. K. Pohl, H. U. Haring, E. D. Schleicher, and C. Weigert Acute regulation of metabolic genes and insulin receptor substrates in the liver of mice by one single bout of treadmill exercise J. Physiol., January 1, 2009; 587(1): 241 - 252. [Abstract] [Full Text] [PDF]