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Single bouts of exercise affect albumin redox state and carbonyl groups on plasma protein of trained men in a workload-dependent manner

Institutes of ¹Physiological Chemistry and ³Physiology, Medical University of Graz; ²Styrian Health Association, Graz; ⁴Institute of Sports Science, Karl Franzens University of Graz, Graz; and ⁵Human Performance Research Graz, Karl Franzens University and Medical University of Graz, Graz, Austria

Submitted 14 December 2007 ; accepted in final form 10 April 2008

The purpose of this study was to investigate the effect of single bouts of exercise at three different intensities on the redox state of human serum albumin (HSA) and on carbonyl groups on protein (CP) concentrations in plasma. Trained men [n = 44, maximal oxygen consumption (O_2max): 55 ± 5 ml·kg^–1·min^–1, nonsmokers, 34 ± 5 years of age] from a homogenous population, volunteers from a police special forces unit, were randomly assigned to perform on a cycle ergometer either at 70% (n = 14), 75% (n = 14), or 80% (n = 16) of O_2max for 40 min. Blood was collected before exercise, immediately after the exercise test (IE), and 30 min after each test (30M) and 30 h after each test (30H). The reduced fraction of HSA, human mercaptalbumin (HMA), decreased at all three exercise intensities IE and 30M, returning to preexercise values by 30H (P < 0.05). HMA was primarily oxidized to its reversible fraction human nonmercaptalbumin 1 (HNA1). CP concentrations increased at 75% of O_2max IE and 30M with a tendency (P < 0.1) and at 80% O_2max IE and 30M significantly, returning to preexercise concentrations by 30H (P < 0.01). These results indicate that the HSA redox system in plasma is activated after a single bout of cycle ergometer exercise at 70% O_2max and 40 min duration. The extent of the HSA modification increased with exercise intensity. Oxidative protein damage, as indicated by CP, was only significantly increased at 80% O_2max intensity in this homogenous cohort of trained men.

albumin modification; carbonylated protein; defined exertion

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