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Developmental effects on myonuclear domain size of rat diaphragm fibers

Departments of ¹Physiology and Biomedical Engineering and ²Anesthesiology, Mayo Clinic College of Medicine, Rochester, Minnesota

Submitted 29 March 2007 ; accepted in final form 8 January 2008

During early postnatal development in rat diaphragm muscle (Dia_m), significant fiber growth and transitions in myosin heavy chain (MHC) isoform expression occur. Similar to other skeletal muscles, Dia_m fibers are multinucleated, and each myonucleus regulates the gene products within a finite volume: the myonuclear domain (MND). We hypothesized that postnatal changes in fiber cross-sectional area (CSA) are associated with increased number of myonuclei so that the MND size is maintained. The Dia_m was removed at postnatal days 14 (P-14) and 28 (P-28). MHC isoform expression was determined by SDS-PAGE. Fiber CSA, myonuclear number, and MND size were measured using confocal microscopy. By P-14, significant coexpression of MHC isoforms was present with no fiber displaying singular expression of MHC_Neo. By P-28, singular expression was predominant. MND size was not different across fiber types at P-14. Significant fiber growth was evident by P-28 at all fiber types (fiber CSA increased by 61, 93, and 147% at fibers expressing MHC_Slow, MHC_2A, and MHC_2X, respectively). The number of myonuclei per unit of fiber length was similar across fibers at P-14, but it was greater at fibers expressing MHC_2X at P-28. The total number of myonuclei per fiber also increased between P-14 and P-28 at all fiber types. Accordingly, MND size increased significantly by P-28 at all fiber types, and it became larger at fibers expressing MHC_2X compared with fibers expressing MHC_Slow or MHC_2A. These results suggest that MND size is not maintained during the considerable fiber growth associated with postnatal development of the Dia_m.

respiratory muscles; postnatal development; skeletal muscle; fiber type; myosin heavy chain

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